1. A cancer drug exploration
Secrets of the Rainforest
A cancer drug exploration

2. The Mysterious Green Florescent Leaves
Hi, I am Tisha
While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal green leaves from Ramon
You bring back to Biotex, a biotech company working on a cure for cancer

3. Green Florescent Protein
Biotex scientists determine that the leaves contain a florescent green protein
DNA from the leaves is inserted into bacterial cells

4. Cloning the Gene from Mysterious Leaves

5. More on Cloning the Gene…

6. Purifying the GFP
Biotex technicians need to separate the GFP from other bacterial proteins
Column Chromatography separates contaminating proteins from GFP
The result is a purified GFP that can be tested for its ability to cure stomach cancer

7. Testing and Placebos
GFP will need to be tested on rats before it is allowed for a human trial
The US Food and Drug Administration will examine Biotex’s experiments to determine if the protein is safe and cures stomach cancer without negative side effects

8. Ethics and Economics
Once GFP is approved by the FDA, you will develop GFP as a commercial product
You will need to be aware of the following viewpoints: FDA, social conscience, patient advocacy groups, and the employees of Biotex
You must resolve the dilemmas in order to successfully market GFP

9. Final Presentations Turn in all lab work with analysis questions
Video Presentation of your marketing of GFP
Address issues of all dilemmas 1-8

10. Background Information- Lesson 1
How to use Pipettes
Streaking of agar plates to Produce Single Colonies (Sterile Technique)

11. Background Continued…Lessons 2 & 3
Process of Transformation (GMOs or genetic recombinants)
Jellyfish , GFP and pGLO and Fluorescence
Isolating Clones to Propagate in Liquid Culture Media

12. Background Continued…Lesson 4
Concentration of Bacteria from Liquid Culture Media

13. Background Continued…Lesson 5
Prepare a Bacterial Lysate for Protein Chromatography

14. Background Continued…Lesson 6
Protein Chromatography- Hydrophobic Interaction Chromatography (HIC)
GFP is hydrophobic and will stick to hydrophobic beads in salt water buffer
When salt is removed, GFP will then be collected
4 buffers:
1) Equilibration buffer (salt buffer)
2) Binding Buffer (high salt)
Wash buffer (medium salt)
Elution buffer (low salt)

15. Background Continued…Lessons 7-10
Lesson 7- testing and placebos
Lesson 8- Advocacy Groups and Viewpoints
Lesson 9-Ethics and Economics
Lesson 10- Preparing and Giving Presentations