1. Impact des mycorhizes sur la revégétation et la succession végétale primaire dans un écosysystème hostile Martina Janoušková, Institute of Botany ASCR, Czech Republic Dr. Diederik van Tuinen, UMR INRA-1088; CNRS-5184; U. de Bourgogne INRA-CMSE, Plant-Microbe- Environment Dijon France

4. PLANTS Tripleurospermum inodorum Calamagrostis epigejos Sisymbrium loeselii AM FUNGI G. intraradices BEG140 G. intraradices BEG141 G. claroideum BEG96 Experimental design

5. PLANTS Tripleurospermum inodorum Calamagrostis epigejos AM FUNGI Non-inoculated G. intraradices BEG140 G. claroideum BEG96 G. intraradices BEG140 + G. claroideum BEG96 10 replicates Harvest at 4 and 8 weeks after inoculation ANALYSES Fresh and dry weight Level of colonisation by AM fungi (Trypan blue) Alkaline phosphatase activity Intensity of root colonisation by each fungus (PCR) Expression of selected fungal genes of G. intraradices (qPCR)

6. Why collaborate? Working on degraded experimental sites. Big collection of AM fungi isolated from degraded environments. Developed molecular tools to monitor fungal gene expression in plant roots, and to detect the fungus in the roots.

7. Results and future Use of the molecular tools to monitor and assess the fungal fitness in field conditions Selection of new markers appropriated for fungal efficiency monitoring Glomus intraradices genome sequencing project