1. Soum Sanogo Department of Entomology, Plant Pathology, and Weed Science, New Mexico State University Aerobiology of Southern New Mexico

2.  Diverse sources: Agricultural and non- agricultural environments.  Winds: Major vehicle for transport from sources to other environments Fungi (molds) in the Air

3.  Outdoors and Indoors While you are taking this quiz, you are probably inhaling fungal spores. Which of the following are least likely to be in the air now in the room? A. Conidia B. Sporangiospores C. Zoospores D. Asexual spores E. All of the above F. None of the above  Concern: load in the air Fungi (molds) in the Air

4. 1)High-volume dust collectors 2)Exposure of petri plates containing growth media outdoors 3)Volumetric air samplers (single-stage and 6- stage Andersen samplers) 4)Rain water collection 5)Sticky tapes mounted on glass slides Sampling Methods

5. Dry deposition (impaction) using volumetric sampler Wet deposition with rain gauge Courtesy DuBois

6. Sampling Methods Single-stage sampler

7. Sampling Methods 6-stage sampler

8. Single-stage sampler Courtesy DuBois

9. Sampling Methods Passive deposition

10. 1)Processing of dust samples impacted upon solid agar media and rainwater samples 2)Identification of fungal microorganisms recovered from dust and rainwater samples Sampling Methods

11. Standard Operating Protocol for Fungal Analysis

12. Sampling locations Las Cruces Palomas Cd. Juarez, Mexico New Mexico Mexico Arizona

13. 1)Concentrated Animal Feeding Operations (CAFOs) 2)Non-CAFOs Sampling locations

14. Aspergillus Aspergillosis

15. Alternaria/ Penicillium/ Fusarium/ Cladosporium Mold allergies

16. Trichoderma/ Rhizopus / Mucor Mold allergies

17. Aureobasidium Allergies/Asthma

21. Predominant Fungi in Rainwater

22.  Fungi  Pollen Other Biological Particulates in Air