1. What are you looking at?

2. What are you looking at now?

3. DNA What are genes made of? Where are genes located? How do they determine characteristics?

4. After reading this, try to answer the questions with your partner… Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms and some viruses. The main role of DNA molecules is the long-term storage of information. DNA is often compared to a set of blueprints or a recipe, or a code, since it contains the instructions needed to construct other components of cells, such as proteins and RNA molecules. The DNA segments that carry this genetic information are called genes, but other DNA sequences have structural purposes, or are involved in regulating the use of this genetic information.nucleic acidgenetic organismsviruses moleculesinformationblueprintscellsproteinsRNAgenes

5. Try to figure out what happened? HarmlessDisease-causing Heat-killed Harmless & Heat- bacteriabacteria disease causingkilled disease causing

6. Disease-causing bacteria (smooth colonies) Harmless bacteria (rough colonies) Heat-killed, disease- causing bacteria (smooth colonies) Control (no growth) Heat-killed, disease-causing bacteria (smooth colonies) Harmless bacteria (rough colonies) Dies of pneumoniaLives Live, disease-causing bacteria (smooth colonies) Dies of pneumonia Section 12-1 Figure 12–2 Griffith’s Experiment Go to Section:

7. Disease-causing bacteria (smooth colonies) Harmless bacteria (rough colonies) Heat-killed, disease- causing bacteria (smooth colonies) Control (no growth) Heat-killed, disease-causing bacteria (smooth colonies) Harmless bacteria (rough colonies) Dies of pneumoniaLives Live, disease-causing bacteria (smooth colonies) Dies of pneumonia Section 12-1 Figure 12–2 Griffith’s Experiment Go to Section:

8. Some History While Frederick Griffith was experimenting with pneumonia, he discovered that mice injected with dead bacteria still died of pneumonia… so it was something inside the bacteria that was still passed on to the next generation. Oswald Avery and other scientists discovered that DNA is the nucleic acid that stores and transmits the genetic information from one generation of an organism to the next.

9. Some More History Alfred Hershey and Martha Chase concluded that the genetic material of the bacteriophage (a virus that “eats” bacteria) is DNA, not protein.

10. Bacteriophage with phosphorus-32 in DNA Phage infects bacterium Radioactivity inside bacterium Bacteriophage with sulfur-35 in protein coat Phage infects bacterium No radioactivity inside bacterium Figure 12–4 Hershey-Chase Experiment Section 12-1 Go to Section:

11. Bacteriophage with phosphorus-32 in DNA Phage infects bacterium Radioactivity inside bacterium Bacteriophage with sulfur-35 in protein coat Phage infects bacterium No radioactivity inside bacterium Figure 12–4 Hershey-Chase Experiment Section 12-1 Go to Section:

12. Bacteriophage with phosphorus-32 in DNA Phage infects bacterium Radioactivity inside bacterium Bacteriophage with sulfur-35 in protein coat Phage infects bacterium No radioactivity inside bacterium Figure 12–4 Hershey-Chase Experiment Section 12-1 Go to Section:

13. DNA Genes are made of DNA = deoxyribonucleic acid How could DNA code for everything genes do? – Genes had to carry information from one generation to the next – Genes had to put that information to work by determining the inheritable characteristics of organisms – Genes had to be easily copied, because it is replicated every time the cell divides

14. DNA’s Structure A long molecule made of units called nucleotides Each nucleotide is made of 3 basic parts: – A 5-carbon sugar called deoxyribose – A phosphate group – A nitrogenous base (There are 4 kinds…) A (Adenine)T (Thymine) G (Guanine)C (Cytosine) PurinesPyrimidines

15. PurinesPyrimidines AdenineGuanine CytosineThymine Phosphate group Deoxyribose Figure 12–5 DNA Nucleotides Section 12-1 Go to Section:

16. A Single DNA Nucleotide Phosphate Group Deoxyribose Sugar Nitrogenous Base

17. DNA Structure Phosphate Group Deoxyribose Sugar Nitrogenous Base Weak Hydrogen Bonds

19. Hydrogen bonds Nucleotide Sugar-phosphate backbone Key Adenine (A) Thymine (T) Cytosine (C) Guanine (G) Figure 12–7 Structure of DNA Section 12-1 Go to Section:

20. The Chargaff “rule” It was discovered that the percentages of guanines (G) and cytosines (C) are almost equal and the percentages of adenines (A) and thymines (T) are almost equal Erwin Chargaff came up with a “rule” that guanine (G) pairs with cytosine (C) and adenine (A) pairs with thymine (T).

21. DNA Base Pairing Nitrogenous base pairs: – Adenine (A) with Thymine (T) – Guanine (G) with Cytosine (C)

22. Let’s pair up DNA! What would pair up with ATCG? TAGC What would pair up with ATTAGC? TAATCG What would pair up with ATACGGTC? TATGCCAG

23. The Double Helix Rosaline Franklin’s x-ray showed that two DNA strands are twisted around each other. James Watson and Francis Crick used Franklin’s x- ray to make a model of DNA. Watson and Crick’s model of DNA was a double helix, in which two strands were wound around each other. Watson and Crick also discovered that hydrogen bonding held the nitrogenous bases together.

25. 2. Which scientists came up with the double helix model of DNA? 1. How many strands does DNA have? a. Hershey & Chase c. Galileo & Aristotle b. Watson and Crick d. Darwin & Malthus a. oneb. two c. threed. four Let’s wrap it up with a few questions together…

26. 3. What holds the strands of DNA together? 5. What DNA bases would pair up with GAATCC? c. CGGAGG a. CTTAGG d. GCCTCC b. GAATCC a. Phosphate Groupb. Deoxyribose Sugar c. Hydrogen Bonds d. Nitrogen bonds 4. What DNA bases would pair up with CATTAT? a. CATTATb. TATTAC c. GTAATA d. GUAAUA

27. Is your answer the same as earlier… What are genes made of?