1. PROCEDURES AND REQUIREMENTS FOR THE SAFE USE OF RADIOACTIVE MATERIAL Indiana University - Bloomington

2. INITIAL AUTHORIZATION TO USE RADIOACTIVE MATERIAL Attend a Radiation Safety Orientation Complete online procedures training Complete and submit a radiation safety exam and application (Form RS-1 or RS-2) Pass “in-lab” safety procedure review

3. ORDERING RADIOACTIVE MATERIAL Indicate user name and PI name on departmental requisition Ensure that shipment address is: Radiation Safety Office - Jordan Hall 071 Plan on being in the lab for notification of the shipment’s arrival (late morning 1-2 days after ordering)

4. RECEIVING RADIOACTIVE MATERIAL Pick up shipment at Jordan Hall 071 Return package to lab and open immediately Follow check-in procedure and complete top portion of Form RS-4 Place source container in secure storage

5. LABORATORY SAFETY RULES Designate and label radioactive material use areas Cover work surfaces with absorbent paper and change frequently Label contaminated equipment and surfaces

6. LABORATORY SAFETY RULES Do not eat, drink, smoke, or apply cosmetics in radioactive material use areas Confine any food and beverages within the lab to designated areas Do not store food or beverages with radioactive materials

7. LABORATORY SAFETY RULES Wear protective apparel including lab coat, safety glasses/goggles, and disposable gloves Do not wear sandals when working with radioactive materials or other chemicals Wear dosimeter properly if assigned

8. CONTAMINATION MONITORING FOR RADIOACTIVE MATERIAL Turn survey meter on, check it’s function, and place it near work area Monitor hands, clothing, and work surfaces throughout procedure Move probe slowly and within a few centimeters of monitored surfaces Decontaminate surface if greater than 3 x BKG

9. CONTAMINATION MONITORING FOR RADIOACTIVE MATERIAL For detection of H-3 (and for monthly surveys for C-14, P- 33, and S-35), wipe surfaces with filter paper (approximately 100 cm 2 per wipe) Analyze wipes in liquid scintillation counter Decontaminate if results are greater than 200 dpm per 100 cm 2

10. RADIOACTIVE WASTE MANAGEMENT Dispose of contaminated items in receptacles specifically designated for radioactive waste. Segregate waste by nuclide, physical form, and chemical content.

11. RADIOACTIVE WASTE DISPOSAL Seal bags and containers; attach completed waste tag to each. Store P-32 waste within the lab (behind shielding) for at least 30 days prior to disposal. Contact RSO to schedule a waste appointment.

12. RADIOACTIVE WASTE DISPOSAL Secure waste on cart (using shielding and secondary containment as needed). Transport waste by cart to waste room. Use freight elevator (not passenger elevator) and tunnels (not outside sidewalks).

13. RADIOACTIVE MATERIAL SPILL RESPONSE Notify persons in the area of the spill Confine the spill with absorbent material Monitor hands, shoes, and clothing for contamination Report spill to RSO Clean-up spill and decontaminate

14. IMMEDIATELY NOTIFY THE RADIATION SAFETY OFFICER OF A major spill (involving the release of > 1 mCi) Any skin contamination or probable uptakes Extensive laboratory contamination (high levels or numerous locations) Any loss of radioactive material

15. SECURITY AND CONTROL OF RADIOACTIVE MATERIAL Lock doors when laboratory is unattended Secure source vials in lockable freezer or refrigerator when not in use Confront strangers in the laboratory

16. REQUIRED RECORDS Keep track of the amount and use of radioactive material through the timely completion of Form RS-4 Return Form RS-4 to JH 071 when material is used (source vial is empty)