1. IRON CONTENT ANALYSIS Due Today: 1. Iron Content Analysis Pre- Lab 2. Neutralizing Power of Antacids Formal Lab Report Homework: 1. Iron Content Analysis Short Report 2. Prepare for Laboratory Final

2.  Introduction to ultraviolet-visible (UV-Vis) spectroscopy  Introduction to analytical chemistry  Determine the iron content in vitamin tablets OBJECTIVE

3. KEY TERMS Visible Range: 700-400 nm UV Range: 10-400 nm

4.  Spectroscopy – measuring the interaction of radiation with matter  Unique wavelength for each substance  Scanning – determine wavelength with maximum absorption  Chemists are most interested in the electromagnetic spectrum between 190-900 nm KEY TERMS

6.  Use Volumetric Glassware  Volumetric flask  Graduated pipets  Burets  Use stock solutions dispensed from burets PROCEDURE NOTES

7.  Wipe off the cuvette with a Kim-Wipe before inserting into the Spectrophotometer  Record the absorbances!  Record the advertised Iron content from both vitamin bottles! PROCEDURE NOTES

8.  Solutions can be disposed of down the drain WASTE

9.  Hydrochloric Acid  Danger  Inhalation Hazard  Corrosive RISK ASSESSMENT

10.  Hydroquinone  Danger  Harmful if Swallowed  Skin Irritant RISK ASSESSMENT

11.  o-phenanthroline  Danger  Toxic if swallowed RISK ASSESSMENT

12. CALCULATIONS

13.  Graphing in Excel  Force the trendline through (0,0)  Include the equation of the tendline and R 2 value CALCULATIONS Y = mx A = bc