1. Name:_______________ Quiz 9: Biol 203 Fall 2013 Lab Instructor Name (1pt):_______________ 26pts total A HD R Arl E1E2E3 4pts. If they own only circle enhancers 1 pt, if they circle some of the gene 2pts, 4pts for everything Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the leg only. 4pts. If they bracket less than this area, then zero points. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna. 4pts. All or none, they have to put the arrow near E1 and E2 or on E3 (destroying it) You want to insert a minimal promoter-Gal4pA transgene near Arl gene. Where is the best place to insert it in order to get leg specific expression and no antenna expression. Using an arrow, point to the location on the Arl gene above. 4pts. You have designed a fancy transgene with a splice acceptor site, the GFP (ATG-stop) gene and a pA. This type of transgene is referred to as a “splice trap”. In order to see GFP expression in the nucleus, where does this transgene need to be incorporated in the above gene?_____after exon 3 within an intron___ _ What else must be true about this incorporation?__GFP has to be in the right reading frame__ means enhancer binding site The Arristaless-like (Arl) gene below is expressed in the leg and the antenna. E1 and E2 are necessary and sufficient to drive expression in the leg. E1, E2 and E3 are necessary and sufficient to drive expression in the antenna. If E3 is deleted, no expression of Arl is found in the antenna. Arl is a transcription factor and thus must get into the nucleus to function. However, the nuclear localization sequence is NOT located on exon 1, exon 2, or exon 4. 1) Does the reverse transcribed first-strand cDNA sequence bind to its mRNA in a anti-parallel direction (yes or no)?_Yes__ 2) (all or none) Name (write out names) for the five most common DNA basesAdenine, thymine, cytosine, guanosine 5methyl cytosine 3) There are four common RNA bases. However, a fifth type of RNA base is involved in wobble position. Name that base. _Inosine__ 4) Pauling and Corey developed a triple helix model that Franklin’s X-ray diffraction data ruled out. What did her data show that ruled out their model?_____bases in or phosphates out, won’t accept double helix_____ 5) Watson and Crick first tried to pair A with A, G with G, C with C and T with T. Why did they try to make these pairs? ___ so that there would be a basis for DNA replication_________________________________. 6) Donohue ruled out the model of Watson and Crick because_Keto form was found in physiological conditions, not enol form of DNA 7) This led Watson and Crick to fix their model in two ways:__A-T, G-C base pairing_______ and ____anti-parallel strands_______.. Q2-6: 1pt per question, Q1 and 7: 2pts each.

2. Name:_______________ Quiz 9: Biol 203 Fall 2013 Lab Instructor Name (1pt):_______________ 26pts total A HD R Arl E1E2E3 4pts. Nothing should have been circled If they circle the whole thing, then 2pts. Circle the portion of the above gene that you would use to make a transgene that expresses Arl in the antenna only. 4pts. If they bracket less than this area, then zero points. Mark with a bracket the portion of the above gene that you would use to make a transgene that expresses Arl in the leg. 4pts. All or none, they have to put the arrow near E1 and E2 or on E3 (destroying it) You want to insert a minimal promoter-Gal4pA transgene near Arl gene. Where is the best place to insert it in order to get leg specific expression and no antenna expression. Using an arrow, point to the location on the Arl gene above. 4pts. You have designed a fancy transgene with a splice acceptor site, the GFP (ATG-stop) gene and a pA. This type of transgene is referred to as a “splice trap”. In order to see GFP expression in the nucleus, where does this transgene need to be incorporated in the above gene?___after exon 3 within an intron___ What else must be true about this incorporation?___GFP has to be in the right reading frame__ ________ means enhancer binding site The Arristaless-like (Arl) gene below is expressed in the leg and the antenna. E1 and E2 are necessary and sufficient to drive expression in the leg. E1, E2 and E3 are necessary and sufficient to drive expression in the antenna. If E3 is deleted, no expression of Arl is found in the antenna. Arl is a transcription factor and thus must get into the nucleus to function. However, the nuclear localization sequence is NOT located on exon 1, exon 2, or exon 4. 1) Does the reverse transcribed first-strand cDNA sequence bind to its mRNA in a parallel direction (yes or no)?__NO_____ 2) (all or none) Name (write out names) for the five most common DNA basesAdenine, thymine, cytosine, guanosine 5methyl cytosine 3) There are four common RNA bases. However, a fifth type of RNA base is involved in wobble position. Name that base. _Inosine__ 4) Pauling and Corey developed a triple helix model that Franklin’s X-ray diffraction data ruled out. What did her data show that ruled out their model?_____bases in or phosphates out, won’t accept double helix_____ 5) Watson and Crick first tried to pair A with A, G with G, C with C and T with T. Why did they try to make these pairs? ___ so that there would be a basis for DNA replication_________________________________. 6) Donohue ruled out the model of Watson and Crick because_Keto form was found in physiological conditions, not enol form of DNA 7) This led Watson and Crick to fix their model in two ways:__A-T, G-C base pairing_______ and ____anti-parallel strands_______. Q2-6: 1pt per question, Q1 and 7: 2pts each.