1. Abundance of the polychaete host of Ceratomyxa shasta and prevalence of infection in the Klamath River. Sue-Jie Koo Mentors: Jerri Bartholomew Sascha Hallett Department of Microbiology Oregon State University

2. Ceratomyxa shasta : The parasite A myxozoan parasite Ceratomyxosis – epithelial lining necrotises in salmonids. Enzootic to the Pacific NW of the United States. –Particularly prevalent in the Klamath River One of the primary causes of juvenile salmonid death in the Klamath River. 54% infected in 2008, compared to 10% in other rivers in the West. ~10 μm

3. Life cycle of C. shasta

4. Manayunkia speciosa : The polychaete Freshwater Definitive host in the lifecycle of C. shasta Few studies on its ecology Spatial distribution Seasonality Density Few studies on its role in the infection dynamics of C. shasta Prevalence of infection Intensity of infection Seasonality Spatial distribution

5. Working hypothesis Polychaete densities and infection prevalence with Ceratomyxa shasta directly correlate with disease effects in salmonids. +=

6. Significance Assist in parasite management options to reduce the impact of the parasite on salmonid populations in the Klamath River. Reduction in returns affect fishermen and farmers Contribute data to the information known about M. speciosa.

7. Methodology The samples were collected as part of a large sampling study in the Klamath River in 2006. R3 R2R1

8. Water samples Sentinel fish studies Beaver Creek R3R2R1

9. Av. # polychaetes x # squares Av. field sampling area Field density = Polychaete Density Stained Manayunkia speciosa

10. Results Beaver Creek R3R3 R2R2 R1R1

11. Prevalence of Infection Results – no parasite detection 630 bp

12. Troubleshooting DNA recovery Parasite spike was inhibited Change DNA extraction buffers and dilution 2006 Polychaete DNA not replicated in PCR Change extraction buffers, dilution, primers, PCR programs Diagnostics comparing 2006 and 2009 polychaetes Crude vs Qiagen purified Qiagen purified 2006 DNA inconsistent results

13. Conclusions Highest polychaete density in highly infectious zone correlates to preliminary studies. 

14. Potential Future Work Manayunkia speciosa temporal distribution M. speciosa prevalence of infection with Ceratomyxa shasta

15. Acknowledgements Sascha Hallett Jerri Bartholomew Gerri Buckles Jill Pridgen Dan Horner Bartholomew Lab Salmon Disease Lab Oregon Department of Fish and Wildlife HHMI Kevin Ahern