2. Making STEM Specimen We will look at each one of these methods in turn.

3. Making STEM Specimen – Dispersion Method

4. Making STEM Specimen – Microtomy Method

5. Making S(T)EM Specimen – Focus Ion Beam (FIB), Ion Mill, Plasma/UV Cleaner Method

6. Making STEM Specimen – Focus Ion Beam (FIB) Method

9. Making a Thin Cross-sectional Specimen by FIB

10. The FIB can weld any two pieces of material together at the nanoscale Welding specimen to 33mm Cu grid

11. Limited Micro/Nano Machining MEMS Lab on a chip

12. Viewing of Cell Cluster

13. Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals

14. Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals Metal deposition

15. Cell Tissue (No Fixation) Samples: Tobacco Flower Petals Courtesy: Kim Findlay, John Innes Centre, Norwich, UK Tobacco flower petals Features never before seen were found in these specimens.

16. Making STEM Specimen – Focus Ion Beam (FIB) Method