2. Introduction to Benthic Macroinvertebrates Trout In The Classroom Virginia Council of Trout Unlimited 2009 / 2010

3. State Water Quality Standards Standards are based on how we use water: 1. 2. 3. Standards include a general requirement that all state waters are free of pollutants harmful to animal, plant, and aquatic life. This standard allows the state to consider stream habitat and aquatic insects as indicators of water quality.

4. Aquatic Insects as Bio- Indicators of Water Quality There are important reasons why benthic macroinvertebrates are used to assess water quality: 1) they are found in all streams; 2) there are many different species; 3) they stay in the same spot in a stream; 4) they react to different conditions in the stream over long periods of time; and 5) they are easy to sample and identify.

5. Benthic Macroinvertebrates Benthic = dwelling on stream bottom Macro = large enough to see with naked eye Invertebrates = without a backbone Other benthic aquatic organisms = aquatic insects that have three body parts (head, thorax, abdominal segment) and six legs When you try to identify an organism, these are some details to notice

6. Six true legs plus lateral filaments Six segmented legs More than six legs prolegs Key ID Features -Legs

7. Key ID Feature – Tails Two tails Feather- like tails No tail or tiny tail Tail-like projection

8. Details to notice: gills Abdominal gills Abdominal filamentous gills Internal gills

9. Practice ID Body shape? Head? Size relative to body? Legs? Number? Length? Placement? Details? Antennae? Eyes? Wings? Tail? Wing pads

10. Specimen #1

11. Specimen #2

12. Specimen #3

13. Specimen #4

14. Specimen #5

15. Specimen #6

16. Specimen #7

17. Specimen #8

18. Specimen #9

19. Specimen #10

20. Specimen #11

21. Specimen #12

22. Specimen #13

23. Specimen #14

24. Specimen #15

25. Benthic Macroinvertebrates can be classified according to their sensitivity to pollutants.

26. Sensitive Mayfly larvae Stone Fly larvae Most caddisfly larvae Beetles (adults and larvae )

27. Somewhat sensitive Dragonfly and damselfly larvae Netspinner caddisfly larvae Crayfish Gilled snails Aquatic sowbugs Scuds Clams and mussels True fly larvae Hellgrammites, Fishfly and Alderfly larvae

28. Tolerant Lunged snails Black fly larvae Midge larvae Aquatic worms Flatworms Leeches

29. Interpreting Benthic Data Benthic macroinvertebrates can be classified according to their sensitivity and tolerance to water pollution.

30. Identify and Tally

31. Individual Species Metric

32. Tolerance Metric

33. Compute Mulitmetric Index

34. Rate the Stream

35. Credits Slides and notes used in this presentation were provided for educational purposes by: Virginia Save Our Streams Virginia Department of Conservation and Recreation Alice Furguson Foundation – Bridging the Watershed Audubon Naturalist Society And a very special thanks to Darrell Schwalm of the Shenandoah Valley TU chapter for putting this presentation together.