1. Introduction to Instruments
FUJI DRI-CHEM 4000i
Scil Vet Focus 5
Cryostat for Frozen Sections
2010/12/06
Speaker：Le-Shi Tsai

2. Notice for the use of animal center Instrument
Please attend illustration meeting before you begin operating instruments. If you do not participate in the illustration meeting , you may NOT use the instrument. (The meeting will be held 2-3 times per semester, and please pay attention to the animal center announcement.)
Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation.
illustration meeting
1 or 2 days prior to use (as the instrument may be) before must be filled Registration forms for the instrument is ready to facilitate the managers
Please fill the registration forms 1 or 2 days prior to use the instrument to facilitate preparation

3. Charging methods Instrument Charging methods $150/0.5hr
FUJI DRI-CHEM 4000i
Biochemistry Analyzer
*Less than 3 tests :
First hour - $ 400/hour
Additional hours-$ 200 /hour
*More than 3 tests :
Each one more test, the first hour charge extra $100
Scil Vet Focus 5
Hematology Analyzer
1 test - $750
each additional test -$100
Leica CM3050 S
Cryostat for Frozen Sections
$150/0.5hr
Less than 3 tests the first hour and $ 400, the beginning of the second hour, $ 200 per hour. More than three hours overcharged 100 yuan more than a first.
Each one more test first hour overcharged 100
Charge $100 in the first hour

4. Biochemistry Analyzer
FUJI DRI-CHEM 4000i
Biochemistry Analyzer

5. Main instrument Need to purchase reagents by user The registration
forms
Exception code
table
Need to purchase reagents by user
Main instrument

6. Colorimetric
Potentiometric
Linear
Measurement time

7. External Structure of the Instrument
Light source
廢料盒
Can not open the lid when indication light is on

8. Internal Structure of the Instrument

9. Automatic detection 1. can be automatically diluted
2. A test only needs at
least 10μL.
（ Manual detection ）

10. Principle：Colorimetric
Spreading layer：
filtered out macromolecules (proteins, dyes, etc.)
evenly spread the fluid sample
into the reaction layer
β-D-glucose＋O2＋H2O GOD D-gluconic acid＋H2O2
1,7-Dihydroxynaphthalene＋4-aminoantipyrine＋H2O2 POD Red dye
505nm
reflection density
calibration curve
glucose concentration

11. Principle：Potentiometric
generated voltage difference between ion selective electrodes applied to a reference solution and to the sample
potentiometric difference between the sample and the reference solution is measured
E = E * RT/Nf * Log ai
ai = f * Ci
Ci = Ion concentration

12. Scil Vet Focus 5
Hematology Analyzer

13. Introduction to Instrument
The following animals can be detected: dog、 rat、 pig、rabbit、mouse、
G. pig
The following items can be detected: WBC、DC、RBC、HGB、HCT、MCV、MCH、MCHC、RDW、Retics、PLT、MPV、PCT
Slide 002

14. Equipment the registration forms the kit printer waste pail
main Instrument

15. Equipment 1.Diluent 2.Clean Deproteinizer 3.Cellyse the kit
1. red blood cell lysis
2. reagent to convert all hemoglobin
to the stable cyanmethemoglobin form

16. Principles 感 應 區 Cells are injected into the core of a
A distinguishing feature of blood cell types is
the diameter of the cells.（RBC、WBC、Platelet）
2. WBCs are classified according to the characteristics of their cytoplasm and nucleus. 3. Cyanmethemoglobin can then be detected by a spectrophotometer set at 540 nm. The concentration of hemoglobin is then calculated from the optical density of the solution. 感 應 區
The cells are forced to pass through aperture in single file (one by one).
Cells are injected into the core of a
sheath flow and confined to a narrow
single-file stream by hydrodynamic
focusing(15μm).
Diluent form a vortex

17. Specimen preparation 1. Use the EDTA anticoagulant 3 ml tubes.
Specimen requirements
1. Use the EDTA anticoagulant 3 ml tubes.
2. Specimen volume: At least 1.5 ml
3. Specimen volume are less than 1.5mL(at least 100 μL):
Use small test tube containing a specimen and add
EDTA as an anti-coagulant (Manual mode).
Preservation conditions
Samples can be analyzed within 4 hour(room temperature).
Interfering factors
Measurement results are affected by hemolysis, chyle and clotted blood.
DO NOT use clotted blood specimen.

18. Automatic Mode open the lid mix the specimen well
put your sample into the
specimen- container
housing rack

20. Cryostat for Frozen Sections
Leica CM3050 S
Cryostat for Frozen Sections

21. Frozen Tissue Processing
tissue process and cutting
fixing and embedding
only use O.C.T as embedding medium
freeze and slice
Hard tissue using a dedicated film
stain
mounting
observe it under a microscope

22. The advantage of Leica CM3050 S
It is ideal for undecalcified hard tissue :
1. can be set to any section thickness and
section automatically
2. with a dedicated film, can avoid the hard
tissue broken.
with rough-cut function
adjustable specimen temperature：
In accordance with the type of specimen to select the appropriate temperature.
adjustable cutting speed

23. Specifications Maximum 40mm 0.5~300μm 0.1~210mm/s -10℃~-50 ℃
Specimen size
Maximum 40mm
Section thickness setting
0.5~300μm
Sectioning speed
0.1~210mm/s
Specimen temperature
-10℃~-50 ℃

24. supplies you need to purchase
Consumables
Suitable specimens
Stage
Buy different size according to the size of the specimen
Disposable blades
General tissue
Ukrainian carbon blades
Hard tissue
Dedicated film

25. Thanks for your attention