1. Slide Preparation D. Crowley, 2007

2. Slide Preparation To be able to prepare a microscope slide Sunday, May 03, 2015

3. Microscope Crossword  Have a go at the microscope crossword - you have five minutes…  Down: - 1.Lens that you look down 2.What the slide is placed on 3.A wheel to make the image clear  Across: - 4.Lens closest to the slide 5.The specimen is placed on this 6.You need this to see something  Have a go at the microscope crossword - you have five minutes…  Down: - 1.Lens that you look down 2.What the slide is placed on 3.A wheel to make the image clear  Across: - 4.Lens closest to the slide 5.The specimen is placed on this 6.You need this to see something Eyepiece Stage Focusing Objective Slide Light

4. Microscopes  Set up a microscope to view an animal cell - remember the steps to take  Place the smallest objective lens (the smallest lens) over the hole in the stage  Turn the coarse focusing wheel to make the gap between the stage and the objective lens as small as possible  Place a slide on the stage, and secure with the clips  Adjust the light source so light goes up through the stage  Turn the coarse focusing wheel slowing, until your specimen is in focus  To see your specimen in more detail, turn the next largest objective lens over your specimen  Use the fine focusing wheel to get your image into focus again - do not use the coarse focusing wheel - this can break your slide!  Set up a microscope to view an animal cell - remember the steps to take  Place the smallest objective lens (the smallest lens) over the hole in the stage  Turn the coarse focusing wheel to make the gap between the stage and the objective lens as small as possible  Place a slide on the stage, and secure with the clips  Adjust the light source so light goes up through the stage  Turn the coarse focusing wheel slowing, until your specimen is in focus  To see your specimen in more detail, turn the next largest objective lens over your specimen  Use the fine focusing wheel to get your image into focus again - do not use the coarse focusing wheel - this can break your slide!

5. Preparing Specimens  A specimen is the object we look at under a microscope  The specimen needs to be thin, so light can pass through it  We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out  When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens: Magnification = magnification eyepiece lens x magnification objective lens  A specimen is the object we look at under a microscope  The specimen needs to be thin, so light can pass through it  We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out  When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens: Magnification = magnification eyepiece lens x magnification objective lens

6. Preparing Onions  You are going to prepare a slide containing a specimen of onion cells  Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification Magnification = magnification eyepiece lens x magnification objective lens  You are going to prepare a slide containing a specimen of onion cells  Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification Magnification = magnification eyepiece lens x magnification objective lens Place a drop of water on the centre of a slide (this may contain a stain to make the specimen more clear, e.g. iodine) Cut some onion, then using forceps, peel off the inside layer of a piece of onion Place your onion skin onto the water droplet on your slide Using a mounted needle, lower a coverslip onto the onion skin. Do this slowly and carefully, to avoid trapping air bubbles!

7. Magnification  When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens: Magnification = magnification eyepiece lens x magnification objective lens E.g. eyepiece lens = 10x objective lens = 60x Magnification = 10 x 60 = 600x magnification  When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens: Magnification = magnification eyepiece lens x magnification objective lens E.g. eyepiece lens = 10x objective lens = 60x Magnification = 10 x 60 = 600x magnification