2. Summary Slide Standards –ScienceScience –MathMath Can you master the microscope? –CluesClues Types of Microscopes Microscope Magnification –Onion Skin CellsOnion Skin Cells –Comparison GraphComparison Graph How to use the microscope –StepsSteps Graphing Time –AnswerAnswer

3. Science Standards S5L3. Students will diagram and label parts of various cells (plant, animal, single-celled, multi-celled). a. Use magnifiers such as microscopes or hand lenses to observe cells and their structure. S5CS3. Students will use tools and instruments for observing, measuring, and manipulating objects in scientific activities. S5CS4. Students will use ideas of system, model, change, and scale in exploring scientific and technological matters. a. Observe and describe how parts influence one another in things with many parts.

4. Math Standards M5P5. Students will represent mathematics in multiple ways. a. Create and use representations to organize, record, and communicate mathematical ideas. c. Use representations to model and interpret physical, social, and mathematical phenomena. M5D1. Students will analyze graphs. a. Analyze data presented in a graph. b. Compare and contrast multiple graphic representations (circle graphs, line graphs, bar graphs, etc.) for a single set of data and discuss the advantages/disadvantages of each. M5D2. Students will collect, organize, and display data using the most appropriate graph.

5. Can you master the microscope? Solve the mystery of the microscope by identifying parts of the microscope from clues provided. Click on the to check your deductive skills. Click on to go on to the next slide.

6. What type of microscope are we using? Microscope Types CompoundDissection Scanning Electron Microscope (SEM) Transmission Electron Microscope (TEM) Compound

7. Clue # 1 These two pieces are used to tilt, lift, support and carry the microscope carefully.

8. Clue # 2 This part provides light to view the specimen.

9. Clue # 3 This knob moves the body tube or stage (depending on your microscope) up and down.

10. Clue # 4 This is the area to place your specimen for viewing.

11. Clue # 5 This part of the microscope regulates the amount of light that enters through the stage opening.

12. Clue # 5 These hold the slide in position.

13. Clue # 7 This piece contains all of the objective lenses.

14. Microscope Magnification Magnification Ocular lens Total magnification Scanning4x10x40x Low power 10x 100x High power 40x10x400x

15. Onion Skin Cells Low-Power High-Power Look at the two pictures below. They are the same onion skin sample shown with the 10x and 40x objective lenses. Create a bar graph that represents the number of whole and partial cells visible at each magnification.

16. Onion Skin Cells Graph

17. Clue # 8 This is a knob that helps to focus on fine details of the specimen.

18. Clue # 9 This is the piece that you look through to see the specimen.

19. Clue # 10 These pieces enlarge the specimen to scanning, low, and high level of magnification.

20. Now we know all of the parts but how do we use the microscope?

21. Step 1 To carry the microscope grasp the microscopes arm with one hand.arm Place your other hand under the base. Place the microscope on a table with the arm toward you.base Plug the microscope in and turn on the light source or adjust the mirror. Make sure that the cords do not obstruct walking paths. light source

22. Step 2 Turn the coarse adjustment knob to lower the stage.coarse adjustment knob stage

23. Step 3 Revolve the nosepiece until the scanning objective lens clicks into place.nosepiece scanning objective lens

24. Step 4 Adjust the diaphragm so that it lets in the most light possible.diaphragm Look through the eyepiece to assure that all parts are correctly aligned.eyepiece

25. Step 5 Place a slide on the stage. Center the specimen over the opening on the stage. Use the stage clips to hold the slide in place.stage clips

26. Step 6 Look at the stage from the side. Carefully turn the coarse adjustment knob to raise the stage until the scanning power objective almost touches the slide.

27. Step 7 Looking through the eyepiece, VERY SLOWLY turn the coarse adjustment knob to lower the specimen until it comes into focus.

28. Step 8 To switch to the low power objective lens, look at the microscope from the side. CAREFULLY revolve the nosepiece until the high-power objective lens clicks into place. Make sure the lens does not hit the slide.low power objective lens Look through the eyepiece and view your specimen. Adjust the fine adjustment knob to improve the focus.

29. Step 8 To switch to the high power objective lens, look at the microscope from the side. CAREFULLY revolve the nosepiece until the high-power objective lens clicks into place. Make sure the lens does not hit the slide.high power objective lens

30. Step 9 Looking through the eyepiece, turn the fine adjustment knob until the specimen comes into focus. fine adjustment knob

31. Step 10 Diagram your observation through each of the objective lenses. Wow…this is huge!

32. Graphing Time Travel around to each microscope and observe its specimen. Keep a tally of living, once living, and non living samples. Work with a partner to create a circle graph that represents the number and percentage of these three types.

33. Answer

34. You are now a…

35. Click here to see if you are correct? Click here to see if you are correct? {After you are finished with the website, click the X in the upper right hand corner of the website.} Clue 1Clue 3Clue 5Clue 7Clue 9 Clue 2Clue 4Clue 6Clue 8Clue 10 Back to

36. Step Step 1 Step 6 Step 2 Step 7 Step 3 Step 8 Step 4 Step 9 Step 5 Step 10 arm base stage course adjustment knob nosepiece scanning objective lens diaphragm light sourcestage clips high-power objective lens Back To Click on the microscope to see the next label. low-power objective lens fine adjustment knob eyepiece