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Unit 3 – Powerpoint #1 Honors Biology 2012-2013
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Use a beam of Light The specimen can be dead or alive
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Have only 1 lens Give two examples of a simple microscope: Magnifying Glass Reading Glasses
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Have more than one ______ lens
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Magnify by 10x or ______ or ________ Shine light on or ____________ the specimen Specimen can be thick or _______. 20x 30x through thin
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Magnify by ______________ Shines light ______________ the specimen Specimen must be _________. 40- 400x thin through
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Use a beam of _______________- The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________ electrons dead air water
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Makes 3D pictures of surfaces Magnify by _________ 60,000x
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Makes _____ pictures of thin slices Magnify by __________ 100,000x 2D
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Butterfly Antenna
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Probe Microscopes Ion Microscopes ◦ _____________ can be seen with these 2 types of microscopes. Atoms
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Horse Hair
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1. Eyepiece Magnifies by 10x
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2. Revolving Nose Piece Changes Objective Lens
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3. Low power Objective Lens Magnifies by 4x (Red)
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4. Medium Power Objective Lens Magnifies by 10x (yellow)
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5. High Power Objective Lens Magnifies by 40x (Blue)
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6. Stage Clips To hold slide in place
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7. Diaphragm Adjusts the amount of light
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8. Light Light to see specimen
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9. On/Off Switch Turns light on and off
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10. Arm To hold while carrying
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11. Stage To set slide on
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To focus only when in Low Power 12. Course Adjustment
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13. Fine Adjustment To focus when using medium or high power
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14. Base To hold while carrying
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A. Carry a microscope with ___________ hands – one hand holding the _______ And the other hand under the ___________ Always hold the microscope upright so the _____________ cannot fall out When you place a microscope at your table, make sure it is _______ from the edge If there is anything wrong with the microscope, or it is damaged in any way, let the _______________ know immediately. B. To clean the objective lenses and eyepiece, use _______________________ BothArm Base Eyepiece Away Teacher Lens Tissue
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1.Prepare your slide 2.Plug the microscope in. Turn on the ______________ 3.Be sure the ___________ Power Objective Lens (_______) is clicked into place. NOTE: Be sure to look at the microscope from the side when changing objective lenses to avoid damaging the ________________ and _____________ 4. Use coarse focus to raise the stage to its ________________ position 5. Place the _______________ on the stage. 6.Look through the eyepiece and turn the __________________ (lowering the stage) until the specimen is visible. 7. Use the ____________ adjustment to bring your specimen into sharp focus. You may wish to move the ________________ to adjust the amount of light. Light Low4X SlideLens Highest Slide Coarse Focus Fine Diaphragm
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8. Make sure the specimen is ______________ 9. Switch to the ___________ Power Objective Lens ( ______) NOTE: Look at the microscope from the ____________ when you change lenses. 10. To avoid losing your focus and/or crushing the slide with the lens, never use the __________________ focus on medium or high power. Use the _____________ focus only to make the specimen visible. 11. Make sure the specimen is ______________. Look from the _________ as you change objective lenses and switch to the __________ Power Lens (________) 12. Use only the _____________ focus to make the specimen visible. 13. When finished, make sure the ___________ power objective lens is in place, wrap the __________ around the _________ of the microscope, cover, and place in the appropriate location Focused Mid10X Side Coarse Fine FocusedSide High40X Fine Low CordArm
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