1. Cheek Cell DNA Extraction

2. DNA Isolation Procedure: 1.Swish your mouth with Gatorade for one minute. Chew lightly on your cheeks. Be careful not to swallow the solution. 2.Spit the solution back into the cup.

3. DNA Isolation Procedure: 3. Carefully pour the spit solution into the 15-mL plastic test-tube provided and screw on the cap 4. With a centrifuge, ask your teacher to spin the down your cheek cells for 30 seconds until it forms into a nice pellet at the bottom of the tube.

4. DNA Isolation Procedure: 5. Use your pipette to carefully draw off most of the liquid on top, without disturbing the cell pellet in the bottom. Leave about 2 mL of liquid.

5. DNA Isolation Procedure: 6. Using a different pipette, add 2mL of lysis buffer to your test-tube. The total amount of liquid in your tube should be 4mL. 7. Screw the cap back on and gently tilt the test- tube back and forth to mix the solution.

6. DNA Isolation Procedure: 8. Using a different pipette, hold your 15mL test tube at a 45⁰ angle, and drip the cold alcohol down the side of the test tube, so that it forms a layer on top of your solution without splashing. Do NOT mix.

7. DNA Isolation Procedure: 9. Let the tube stand upright and undisturbed. You should begin to see clear strands of DNA clumping together in the alcohol layer.

8. DNA Isolation Procedure: 10. With your pipette, carefully transfer the precipitated DNA along with about 1 ml of the alcohol solution into the small test tube that you will have for your necklace.