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BIO 225 Microbiology Ken Malachowsky

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Presentation on theme: "BIO 225 Microbiology Ken Malachowsky"— Presentation transcript:

1 BIO 225 Microbiology Ken Malachowsky
Florence-Darlington Technical College

2 MICROSCOPE SPECIMEN Magnifies Illuminates Contrasts Resolves Optics
Distinguishes Lens systems Background Ability to Vary Light intensity Condenser OCULAR OBJECTIVE Provide sharp, crisp image Lamp Distinguish Total magnification Two close objects Iris diaphragm Multiply two powers

3 Leewenhoek’s Simple Microscope

4 A Compound Brightfield Microscope

5 Scanning Power X 4x

6 Low Power X X 4x 10x

7 High Power X X 4x 10x 40x 4x 10x 40x 100x Field of Vision

8

9 Depth Perception Steve Berg winona state university, used with
permission

10 Refraction

11 The use of Immersion Oil

12 Microscope Rules Never force anything on the microscope
Inform the instructor if there is a problem with the microscope Only use your assigned microscope Please do the following when you are finished Click the 4x objective in place Lower the stage far from the objective Place the microscope in its assigned space

13 Slides to View Letter E Colored Threads Cheek cells
Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

14 Cheek Cells 100x 400x KM

15 Slides to View Letter E Colored Threads Cheek cells
Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wetmount KM

16 Cyclops 100x KM

17 Daphnia 100x KM

18 Volvox 100x 400x KM

19 Spirogyra 400x KM

20 Amoeba 100x 400x KM

21 Paramecium 100x 400x KM

22 Representative algae, or plantlike protists: The diatom Campylodiscus hibernicus
These protists usually have cell walls surrounded by a loosely attached test that contains silicon or calcium carbonate. Most reproduce by binary fission. Some lack flagella.

23 Diatoms 400x KM

24 Mixed Bacteria 1000x

25 Slides to View Letter E Colored Threads Cheek cells
Arthropod: Cyclops or Daphnia Algae: Volvox, Spirogyra, or Stentor Prototists: Amoeba, Paramecium or diatoms Mixed Bacteria Pond water wet mount

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