1. Identification of Mitochondria in Plant Cells Frey Scientific Cellular World Module Unit 2 Activity 3 Kasi Bolden & Susan Rathwick

2. Helpful introduction to cells http://www.cellsalive.com/cells/cell_model.htm http://www.cellsalive.com/cells/cell_model.htm http://www.cellsalive.com/cells/cell_model.htm http://www.biologycorner.com/worksheets/cells alive.html http://www.biologycorner.com/worksheets/cells alive.html http://www.biologycorner.com/worksheets/cells alive.html http://www.biologycorner.com/worksheets/cells alive.html

3. Objective Students will identify mitochondria and other plant cellular organelles Students will identify mitochondria and other plant cellular organelles

4. When to do this lab? During the study of the cell During the study of the cell Once students have become familiar with the structure and function of cellular organelles, including mitochondria Once students have become familiar with the structure and function of cellular organelles, including mitochondria

5. Materials 1 Celery stalk 1 Cover slip 1 Cover slip Forceps Forceps Janus Green B vital stain Janus Green B vital stain 1 Microscope, compound 1 Microscope, compound light light 1 Microscope slide 1 Microscope slide Paper towels Paper towels 1 Pipette, plastic 1 Pipette, plastic Apron Apron Gloves Gloves Safety goggles Safety goggles

6. Procedure Make a wet-mount with celery using 5% sucrose solution and view plant cell organelles under the microscope. Make a wet-mount with celery using 5% sucrose solution and view plant cell organelles under the microscope. Add Janus B Green stain to enhance view of mitochondria. Add Janus B Green stain to enhance view of mitochondria.

7. Discussions prompted from observations Function and or review of organelles Function and or review of organelles Reason for both chloroplast & mitochondria in a plant cell Reason for both chloroplast & mitochondria in a plant cell Endosymbiotic theory Endosymbiotic theory

8. Is this a beneficial activity?