1. Protein mix, in-sol digestion, LCMSMS Run II, Skupina 1 MS, TIC

2. Protein mix, in-sol digestion, LCMSMS MS, TIC Run II, Skupina 2

3. Protein mix, in-sol digestion, LCMSMS MS, TIC Run II, Skupina 3

4. Protein mix, in-sol digestion, LCMSMS MS, TIC Run II, Skupina 4

5. Protein mix, in-sol digestion, LCMSMS MS, TIC Run II, Skupina 5 PEGs

6. MS, TIC

7. MS spektrum 3+ 2+ MW3+ 772.87 x 3 – 3 = 2315.6 2+1158.65 x 2 – 2 = 2315.3

8. EIC Extracted ion chromatograms (MS) m/z 904.5 m/z 773.1 MS, TIC

9. m/z 904.5 m/z 773.1 MS/MS spektrum

10. 1. gi|30794348 Mass: 24513 Score: 545 Queries matched: 28 emPAI: 2.59 casein alpha s1 [Bos taurus] 772.7830 2315.3272 2315.1296 0.1976 0 82 3.3e-005 K.EPMIGVNQELAYFYPELFR.Q 4. gi|27806963 Mass: 26002 Score: 286 Queries matched: 7 emPAI: 0.62 casein alpha-S2 [Bos taurus] 903.8650 2708.5732 2 708.4003 0.1729 0 68 0.00075 K.FPQYLQYLYQGPIVLNPWDQVK.R Mascot

11. #bb ++ b*b* ++ b0b0 b 0++ Seq.Yy ++ y*y* ++ y0y0 y 0++ # 1130.049965.5286 112.039356.5233E 19 2227.1026114.0550 209.0921105.0497P2187.09431094.05082170.06781085.53752169.08381085.045518 3358.1431179.5752 340.1326170.5699M2090.04161045.52442073.01501037.01112072.03101036.519117 4471.2272236.1172 453.2166227.1119I1959.0011980.00421941.9745971.49091940.9905970.998916 5528.2486264.6280 510.2381255.6227G1845.9170923.46211828.8905914.94891827.9064914.456915 6627.3171314.1622 609.3065305.1569V1788.8955894.95141771.8690886.43811770.8850885.946114 7741.3600371.1836724.3334362.6704723.3494362.1783N1689.8271845.41721672.8006836.90391671.8166836.411913 8869.4186435.2129852.3920426.6996851.4080426.2076Q1575.7842788.39571558.7577779.88251557.7736779.390512 9998.4612499.7342981.4346491.2209980.4506490.7289E1447.7256724.36651430.6991715.85321429.7151715.361211 101111.5452556.27621094.5187547.76301093.5347547.2710L1318.6830659.84521301.6565651.33191300.6725650.839910 111182.5823591.79481165.5558583.28151164.5718582.7895A1205.5990603.30311188.5724594.78991187.5884594.29789 121345.6457673.32651328.6191664.81321327.6351664.3212Y1134.5619567.78461117.5353559.27131116.5513558.77938 131492.7141746.86071475.6875738.34741474.7035737.8554F971.4985486.2529954.4720477.7396953.4880477.24767 141655.7774828.39231638.7509819.87911637.7668819.3871Y824.4301412.7187807.4036404.2054806.4196403.71346 151752.8302876.91871735.8036868.40541734.8196867.9134P661.3668331.1870644.3402322.6738643.3562322.18175 161881.8728941.44001864.8462932.92671863.8622932.4347E564.3140282.6606547.2875274.1474546.3035273.65544 171994.9568997.98211977.9303989.46881976.9463988.9768L435.2714218.1394418.2449209.6261 3 182142.02521071.51632124.99871063.00302124.01471062.5110F322.1874161.5973305.1608153.0840 2 19 R175.119088.0631158.092479.5498 1 EPMIGVNQELAYFYPELFR