1. Alternative additives
Eugenio Dr.Vincenzi
Technical Manager Sintofarm

2. SintoMANN
SintoMANN is a prebiotic rich in mannanoligosaccharides (MOS), produced from the cell wall of a specially selected strain of Saccharomyces cerevisiae.
Highly efficient in the agglutination of pathogens, reducing risk of pathogen colonization of gut.
Modulates gut flora, with reduction of gut disorder episodes and negative impact on animals.
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3. SintoMANN
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4. Hydrolysis – High solubles, added minerals (clay), loss of mannans
SintoMANN
Competition Products – A Few Results
Innactive Dry Yeast – Solubles due to wall rupture caused by fast water evaporation
Partial ‘Lysis’ – Low protein suggests separation of extracts to some extent, but still too much solubles
Hydrolysis – High solubles, added minerals (clay), loss of mannans
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5. SintoMANN
Average (log 10) of counts of SALMO in cecal content of broilers (3 and 6 days after infection)
Furlan e Berchieri Jr., 2008
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6. SintoMANN
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7. Layered Cell Wall Structure
SintoMANN
E.coli pili attaches to SintoMANN
Yeast Cell Wall
E.coli
Layered Cell Wall Structure
Yeast
Cell Wall
E.coli
pili
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8. Don´t produce type 1 fimbriae
SintoMANN
SintoMANN agglutinates isolated strains of Vibrio ordalii and atypical Aeromonas salmonicida
¹ Bacteria were cultivated in Luria agar and TSA agar (Tripticase Soy agar).
² After incubation, 2 bacterial suspensions were produced: 0.25 (7.5X107) and 0.50 McFarland scale (1.5X108 bacteria/ml).
Negative(-): absence of agglutination reaction.
Mild reaction (+): focal agglutination reaction
Moderate reaction (++): diffuse agglutination reaction.
Don´t produce type 1 fimbriae
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9. SintoMANN SintoMANN works in the pathogen agglutination.
Experimental Trial in vitro (Santurio, J. M.; Alves, S. M Universidade Federal de Santa Maria).
SintoMANN agglutinated pathogens GRAM -
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10. SintoMANN SintoMANN acts on the reduction of Clostridium perfringens.
8% Lower Mortality
Positive Synergy with Antibiotic GRAM+
Experimental trial carried out with broilers challenged with Clostridium perfringens, added SintoMANN (1,5 kg/ton).
(Mathis, G.F. et al Southern Poultry Research, Inc. Athens, GA).
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11. 14% Better Feed Conversion
SintoMANN
LSD Test (P<0,05)
14% Better Feed Conversion
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12. SintoMANN 18% Higher Weight Gain Eugenio Dr.Vincenzi Sintofarm spa
LSD Test (P<0,05)
18% Higher Weight Gain
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13. SintoMANN SintoMANN acts promotes intestinal health
SintoMANN increased the number of Bifidobacteria.
Experimental trial carried out with broilers, with dietary inclusion of SintoMANN in the starter and grower diets(1.5 and 1.0 kg/ton, respectively). (Bushansingh, B.; Zhao, X McGill University, Montreal, Canada).
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14. SintoMANN improved the integrity of the intestinal mucosa.
1 SEM (Standard Error of the Mean): Mean ± SEM of 9 replicates).
Test of Scheffe (P>0.05)
SintoMANN improved the integrity of the intestinal mucosa.
Eugenio Dr.Vincenzi Sintofarm spa

15. SintoMANN allows the withdrawal of antibiotic growth promoter
Tukey Test (P<0,05)
Experimental trial carried out with broilers, with dietary inlcusion of SintoMANN in pre-starter / starter, grower and finisher diets (1.5, 1.0 and 0.5 kg/ton, respectively)..
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16. SintoMANN
Tukey Test (P<0,05)
SintoMANN was better than competitors in jejunum villi height and weight gain.
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17. SintoMANN improves performance
Duncan Test (P<0,05)
Experimental trial carried out with broilers, with dietary inclusion of SintoMANN in pre starter/starter, grower, and finisher diets, (1.5; 1.0 and 0.5 kg/ton, respectively).
Eugenio Dr.Vincenzi Sintofarm spa

18. Better Feed Conversion
SintoMANN
Duncan Test (P<0,05)
SintoMANN promoted:
Weight Gain
Better Feed Conversion
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19. SintoMANN Assessment of SintoMANN additioned to broiler diets
Improves on average the production
by 3.3%.
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20. SintoMANN promotes weight gain in piglets 14% Better Feed Conversion
18% Higher Weight Gain
14% Better Feed Conversion
10% Higher Body Weight
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21. SintoMANN
SintoMANN controls the incidence of diarrhea of newly – weaned piglets
Fecal score of piglets fed different levels of a prebiotic, source of mannanoligosaccharides (SintoMANN)
- 23,3% of diarrhea with 1kg/MT of SintoMANN
- 47,5% of diarrhea with 2kg/MT of SintoMANN
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22. SintoMANN
SintoMANN increases the production of acetic acid and propionic in dogs
Short-chain fatty acids, derived from the metabolism / differential fermentation of certain sugars, are favorable for increasing the production of energy available for the maintenance of intestinal integrity.
SintoMANN increased the concentration of butyric acid, linear and showed superior effect on the production of acetic acid and propionic.
Eugenio Dr.Vincenzi Sintofarm spa

23. SintoMANN
SintoMANN promotes reduction of tyramine and histamine in feces of dogs
SintoMANN promoted linear reduction in the concentrations of tyramine and histamine in feces and resulted in responses in the fecal production of phenyl ethylamine.
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24. SintoMANN promotes adherence of E.coli
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25. SintoMANN SintoMANN promotes adherence of E.coli
SintoMANN is higher in agglutination of bacteria E. coli.
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