1. SafetySafety Farshid Karbassian By courtesy of Ramin Jamnejad

2. OutlineOutline Signs and Labels Signs and Labels Chemical Fume Hood Chemical Fume Hood Labware Labware Personal Protective Equipment (PPE) Personal Protective Equipment (PPE) 2

3. Signs & Labels Before starting an experiment, know all the physical, chemical, and toxicological properties of all the materials with which you will be working with. Before starting an experiment, know all the physical, chemical, and toxicological properties of all the materials with which you will be working with. 3

4. Signs & Labels 4

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6. Chemical Fume Hood 6

7. Work at least 10 cm Work at least 10 cm inside the hood. Lower the sash to the Lower the sash to the lowest practicable position. 7

8. Chemical Fume Hood Keep hood surfaces clear and baffles unobstructed to allow proper airflow. Keep hood surfaces clear and baffles unobstructed to allow proper airflow. Airflow in a fume hood is easily disrupted; do not use the fume hood for storage! 8

9. Chemical Fume Hood Do not lean into the Do not lean into the hood or you will expose yourself to harmful fumes. Stay behind the sash. Stay behind the sash. 9

10. Chemical Fume Hood Do not put yourself in a Do not put yourself in a position that increases the risk of chemical exposure. Putting your face at sink top Putting your face at sink top level is dangerous: – Splash hazard – Inhalation hazard Do not rest your hands/arms on the sink surface. Do not rest your hands/arms on the sink surface. 10

11. LabwareLabware Make sure that the labware Make sure that the labware you use is compatible with the chemicals (or mixtures) you pour into them. Example: HF will attack a Example: HF will attack a glass container.  Rule of thumb: Use labware made out of the same type of material that the chemical is stored in. 11

12. Personal Protective Equipment (PPE) The following PPE is required while using acids in any hood: Eye protection Eye protection Gloves Gloves Face shield Face shield Apron Apron 12

13. Personal Protective Equipment (PPE) When working near someone working with chemicals, around chemicals, but not directly with chemicals themselves, always wear some form of eye protection! 13

14. Personal Protective Equipment (PPE) Glove materials (PVA nit rile, butyl, PVC) show different degrees of resistance to chemicals. Heavier gloves provide more chemical resistance, but less dexterity. Thin exam gloves, especially Latex provide almost NO chemical resistance. 14

15. Personal Protective Equipment (PPE) Labcoats provide extremely limited but critical protection from chemical splashes and is useful when working with small quantities of hazardous chemicals. Heavily contaminated or well-worn labcoats must either disposed of or laundered, before they become sources of exposure themselves. 15

16. Buddy System Never work alone. Two or more people must be in the lab at any time. 16

17. Any questions?