1. Name Institution Education Field of interest Address Phone E-mail Further Details: Department of Microbiology and Molecular Genetics Quaid-e-Azam Campus, University of the Punjab Lahore-54590 Pakistan Phone: +92 42 35952811,33 Fax: +92 42 99230481 E-mail: imran.mmg.pu.edu.pk Photocopy can be used or download from University of the Punjab website. Registration fee is Rs 1000/- in cash. Maximum 25 participants will be selected Please completely fill the form and submit to address below by hand, by mail or email. Dr. Imran Sajid Department of Microbiology and Molecular Genetics Quaid-e-Azam Campus, University of the Punjab Lahore-54590 Cell: O333-4730704 Imran.mmg@pu.edu.pk imransajid80@yahoo.co.uk Bacterial Endotoxin Testing (LAL Test) Organized by Department of Microbiology and Molecular Genetics, In Collaboration With Pharm Canada Pakistan Wednesday, June 20, 2012 09:00AM – 04:00PM Department of Microbiology and Molecular Genetics, University of the Punjab Lahore Department of Microbiology and Molecular Genetics, is a distinguished institute in the University of the Punjab academically and with reference to research facilities. Patron: Prof. Dr. Shahida Hasnain Distinguished National Professor Chairperson: Prof. Dr. Anjum Nasim Sabri Organizing Secretory: Dr. Imran Sajid Members: Dr. Basharat Ali Dr. Mahboob Ahmed Mr. Numan Javed Mr. Yasir Rehman  1 st Prize wining image, Nikon Small world Competition 2010 Registration Deadline Tuesday 19 June, 2012 One Day Workshop on

2. Limolus Amoebocytic Lysate Test (LAL Test) is a premier method to detect the lipopolysaccharides (LPS) called endotoxins in Gram negative bacterial cell wall and has a wide spread industrial application, especially in pharmaceutical industry. High concentration of lipopolysaccharides in pharmaceutical preparations like antibiotics etc may have severe health problems such as: pyrogenecity, change in blood composition and shock. Different techniques have been developed to detect the presence of endotoxins in the pharmaceutical preparations, however LAL test is considered as the most authentic and most commonly used technique for this purpose. Young researchers & post graduate scholars from public & private sectors universities, research institutes and colleges involved in the microbiology especially in pharmaceutical microbiology, chemistry and pharmacy Registration08:30 -09:00 Welcome & Introduction of Participants 09:00 -09:15 Session 109:15 - 10:15 Bacterial Endotoxins: Origin, Composition and Pharmacological effects Dr. Imran Sajid (MMG) Tea Break: 10:15 - 10:45 Session 210:45 - 01:00 Testing Methodologies: LAL test (Gel clot method, chromogenic method, turbidimetric method) Mr. Shafat Ali (Pharm Canada Pakistan) Lunch & Prayer Break 01:00 - 02:00 Session 3 Practical Session 02:00 - 04:00 Dr. Imran Sajid Mr. Shafat Ali Mr. Yasir Rehman Mr. Usman Aftab Mr. Asif Raheem Qiasrani Understanding the nature and pharmacological effects of endotoxins Having an overview of the testing methodologies used in endotoxin testing Practicing the LAL test: gel clot method and related techniques