Download presentation
Presentation is loading. Please wait.
Published byLily Lowry Modified over 10 years ago
1
RFLP Restriction Fragment Length Polymorphism Marie Černá, Markéta Čimburová, Marianna Romžová
2
RFLP –Restriction Fragment Length Polymorphism restriction analysis of DNA by its digestion with restriction endonucleases (RE) in specific restriction sites
3
RFLP –Restriction Fragment Length Polymorphism in the case the sequence difference (polymorphism) creates or disturbs a specific site for RE, after restriction, fragments with different sizes are formed
5
Principle starting DNA (genomic DNA, PCR product) digestion with a restriction enzyme into fragments with different sizes fragments electrophoresis separation
6
Restriction endonucleases (RE) known about 2100 bacterial RE RE recognize variously short nucleotides sequences (4,6,8), in which then they digest covalent phosphodiester bonds
7
??? Do RE often digest, which recognize shorter or longer sequences? What is a role of RE in natural systems?
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.