1. Chanleakhena Phoeung, Aung Naing Myo, Stéphane Bayen, Boon Huan Tan, Nicolas Steenkeste, Khov Meas, Puy Lim, Monidarin Chou, Barry Kelly Symposium on Biodiversity & Health Symposium 17-18 th November 2014

2. Outline Introduction Exposure to Pesticides Objective and Method of study Results of study Conclusion Recommendations

3. Source : Nile River Awareness Kit "Physical and Chemical Characteristics of Water Quality."Physical and Chemical Characteristics of Water Quality Introduction

4. Problems bioaccumulation and Biomagnification

5. Detection the presence of frequently used pesticides in fish collected from Tonle Sap lake in Cambodia Target compounds : 65 POPs – Organochlorines (OCPs) – Polychlorinate biphenyls (PCBs) – Bis-(p-chlorophenyl) sulfone (BCPS) – …… Objective of Study

6. Sampling sites : Siem Reap, Pursat, K. Thom, K. Chhnang 76 fishes collected 36 samples analyzed -12 (12 species)from Siem Reap -15 (12 species)from Pursat -4 ( 4 species) from K. Thom -5 (5 species) from K. Chhnang Study sites and sample collection TS1 TS2 TS3 TS4 Chong Kneas Phat Sanday KamPong Luong Chnok Trou

7. Gel permeation chromatography (GPC) with organic solvents followed by Florisil Clean-up Samples processing

8. Injector : splitless mode Gaz : He Column : DB- 5 MS 60 m X 0.25 mm X 0,10 µm T ˚ column : 80˚ C Inj. Vol : 3 ml/min, T ˚ inj : 250 ˚C Flow rate : 1,2 ml/min Presure : 155,7 kPa 1min 80˚C 160˚C 20 ˚C/min 200 ˚C 4 ˚C/min 250 ˚C 2 ˚C/min 20 ˚C/min 10min Gradient 300˚C 8 GAS CHROMATOGRAPHY-MASS SPECTROMETRY (GC-MS) : Condition Samples Analysis

9. RESULTS

10. Samples characteristic To tal TS1 463133331211000031 TS2 0101000310001033022 TS3 0011300010000101017 TS4 11110100100000006 To tal 5854643642111043 76 10

11. Main POPs detected Compound nameConcentrationPermissible limit p,p’- DDT0.00 to 3.70 ng/g5ppm PCBs0.00 to 9.99 ng/g2ppm BCPS0.00 to 0.86 ng/g10ppm FAO J Toxicol Environ Health A. 1999 Jun 11;57(3):185-98. J Toxicol Environ Health A.

12. Result of DDT Samples Concentration (ng/g)

13. Result of PCBs Samples Mean of Concentration (ng/g)

14. Result of BCPS Concentration (ng/g) Samples

15. POPs detected in different sites Study sites

16. Conclusion Trace of dangerous compounds are detected in Tonle Sap fish Predominant compounds are p,p’-DDT, PCBs and BCPS. Concentration of each compound is variable from one site to another These compounds are bioaccumulated and biomagnified it may affect to the people. Limit use of dangerous pesticides by educating users about its hazard Monitor residue frequently of pesticides in ecology system

17. Acknowledgements