1. Histology Laboratories Molecules to Systems 2003 Compiled by James D. Jamieson, MD/PhD Thomas L. Lentz, MD No part of this image collection may be distributed outside of the Yale University Intranet.

2. Alberts, B. et al. Molecular Biology of the Cell. 4th Edition, Garland Science, New York, 2002. Gartner, L. P. and Hiatt, J. L. Color Atlas of Histology, Williams & Wilkins, Baltimore, 1994. Kerr, J. B. Atlas of Functional Histology. Mosby, London, 1999. Kessel, R. G. and Kardon, R. H. Tissues and Organs: a text-atlas of scanning electron microscopy. W. H. Freeman, San Francisco, 1979. Lentz, T. L. Cell Fine Structure. W. B. Saunders, Philadelphia, 1971. Lodish, H. et al. Molecular Cell Biology. W. H. Freeman, New York, 2000. Mizoguti, H. Color Slide Atlas of Histology. Nihon Shashin Shinbunsha, Tokyo. Young, B. and Heath, J. W. Wheater’s Functional Histology. Churchill Livingstone, Edinburgh, 2000. Micrographs taken by George Palade, Marilyn Farquhar, James D. Jamieson, Nicolai Simionescu, Maya Simionescu, David Castle, Thomas L. Lentz. Web Resources http://info.med.yale.edu/webpath/webpath.htm Cushing Library Educational Software/Cell Biology/Several Histology Resources Acknowledgements Sources of Micrographs, Diagrams and Figures

3. Bone Laboratory

4. Adult Bone

5. Some Useful Terms Compact bone = cortical bone Trabecular bone = spongy = cancellous bone Osteoid: Bone matrix not yet calcified. Woven bone: immature bone with randomly arranged collagen fibers. Produced when osteoblasts rapidly produce osteoid as in fetal bone development; fracture repair (callus). Lamellar bone: regular parallel bands of collagen. Woven bone eventually remodeled into lamellar bone. The normal form of bone in adults.

6. Compact bone Trabecular bone

7. Cortical Bone Volkmann’s canals Haversian canals Lamellar bone

8. Haversian canal Osteon: Lamellar Bone Osteocytes Canaliculi

9. Osteocytes in Lacunae in Lamellar Bone

10. Trabecular Bone

11. Trabecular Bone: XS Long Bone Muscle

12. Trabecular Bone Trabeculae Marrow Periosteum Endosteum

13. Periosteum Fibrous layer Cellular layer Compact bone Osteoclasts

14. Bone Marrow Endosteum

15. Articular Surface of Long Bone Cartilage Bone Collagen layer

17. Bone Formation

18. Intramembranous Ossification (No cartilage model; bones of skull)

19. Intramembranous Ossification: Fetal Skull Osteoclasts Osteoblasts

20. Osteoclasts Osteoblasts Intramembranous Ossification: Fetal Skull

21. Endochondral Ossification (Ribs, long bones, vertebrae; cartilage model)

22. Endochondral Bone Formation

23. Fetal Finger Epiphyseal growth plates (GP) Diaphysis Epiphysis (E) Epiphysis

24. Epiphysis With Trabecular Bone GP E Metaphysis

25. Growing Tibia GP E Articular cartilage M D

26. GP Bone Calcified cartilage Osteoid Growing Tibia

27. Chondrocyte Growth Sequence Resting Proliferation Hypertrophy Maturation Zone of calcifying cartilage

28. Development of Trabecular Bone Calcified cartilage Bone Osteoblasts; osteoid

30. Bone Remodeling Activation Resorption Formation Sequence

31. Osteoclasts Howship’s lacuna

32. Osteoblasts (OB) Osteoid Vessels in Resorption Canal Osteocytes in new lamellar bone

33. Resorption Canal XS OB Osteoid

34. Some pathology to think about.

35. Mammary Carcinoma Metastases to Vertebra

36. Callus at Fracture Site

37. New Bone at Fracture Site

38. Osteoporosis with Vertebral Collapse

39. Osteogenic Sarcoma/Osteoid