1. Hyaluronic Acid Hydrogel Films October 27, 2008 Scott Zawko – Post-doctoral Researcher (Presentation to Dr Raymond Grill)

2. Isolation of Hyaluronic Acid - Hydrophilic -High Molecular Weight -Viscoelastic Solutions - Anionic Molecular Properties 1934 - HA isolated from cattle eyeballs

3. Other Applications - Adhesion Barrier (Seprafilm ® ) - Dermal Filler (Restylane ® ) Hyaluronic Acid Materials Images taken from healon.com and synvisc.com Vitreous substitute (Healon ® ) Viscosupplementation (Synvisc ® )

4. + GMHA Triethylamine Water/Acetone (50/50) Synthesis of GMHA Hyaluronic Acid (HA) Glycidyl Methacrylate (GM) The average degree of substitution of GMHA is 0.32 (i.e., 32 out of every 100 disaccharides bears a methacrylate group).

5. 365 nm UV Irgacure 2959 Photocrosslinked GMHA Hydrogels GMHA Bulk HydrogelsGMHA Hydrogel Film photocrosslink

6. GMHA Bulk Hydrogel Biocompatibility Leach, J.B. et al. 2003 Biotechnol. Bioeng. EXPERIMENTAL: Hydrogels were subcutaneously implanted in rat and explanted at 2 weeks. The implanted hydrogels had been placed in plastic devices that allowed them to be easily located for explantation. RESULTS: Inflammation was minimal; hydrogels were partially degraded; endothelial cell infiltration into GMHA hydrogels was not statistically different from fibrin gels.

7. Features of GMHA Hydrogel Films Optically Transparent Low Swelling (66% water) Non Cell Adhesive Zawko, S.A. Dissertation, UT Austin

8. GMHA hydrogel films as a surgical nerve wrap - Prevent cellular infiltration - Permit diffusion of oxygen, salts, metabolites - Biodegradable - Isolate nerve gap

9. Scale-up of crystal templated HA hydrogels 1000  m Scale bars are 10  m Zawko, S.A. Dissertation, UT Austin

10. Schmidt Lab Publications with GMHA Hydrogels 1. Chemistry and material characterization 2. Peptide functionalization 3. Protein release Leach, J.B. et al. 2003 Biotechnol. Bioeng. Leach, J.B. et al. 2004 J. Biomed. Mater. Res. Leach, J.B. et al. 2005 Biomaterials 4. Drug release Zawko, S.A. et al. (In press) J. Biomed. Mater. Res. 5. Patterned swelling Zawko, S.A. et al. (In press) Acta Biomaterialia 1. Synthesis and characterization Zawko, S.A. Dissertation, UT Austin GMHA Bulk Hydrogels GMHA Hydrogel Films 2. Crystal templating Zawko, S.A. Dissertation, UT Austin (Manuscript in preparation)