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Published byAspen Hodnett Modified over 9 years ago
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Přenosy jader J. Fulka, Jr. CBTTN a VÚŽV Praha
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Fertilization – transformation of meiotic cells into mitotic cells Gametes – terminally differentiated and highly specialized Genomes - (oocyte, sperm) transcriptionally inactive after fertilization One cell stage embryo is totipotent Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES
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A)Sheep B)Mouse C)Human D)Rabbit E)Bovine F) Pig G) Monkey
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Mouse embryo Human embryo PB MPN FPN MPN 5-MeC
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Human embryo
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PB PN MPN FPN 49% of human 1-cell stage embryos showed abnormal demethylation pattern
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2PB FP MP
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Wilmut, I et al. (1997) Viable offspring derived from fetal and adult mammalian cells. Nature 385, 810-813
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A B AB
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Enukleace
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Přenos jádra
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What do we need??
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Oct4Cdx2 DAPI merge
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Nanog
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Oct4DAPI
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Differentiation βIII tubulin TROMA 1 DAPI MF20 + EB formation
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Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň
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Pacient ESc linie
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Producing primate embryonic stem cells by somatic cell nuclear transfer Nature - doi:10.1038/nature06357 …. potřebujeme vůbec přenosy jader?
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Děkuji za pozornost
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