1. OBJ. 4 MAB PIPELINE ESTABLISHMENT & IMPLEMENTATION Year 3 Deliverables, Challenges, Year 4 Goals

2. Goal What Impact Integrate modern genomics tools with traditional breeding approaches, systematically (8-stage MAB Pipeline) Enrich breeding material for favorable genetics to efficiently increase likelihood and accuracy of identifying top performers

3. Year 3 Deliverables What Many Jewels available, some already in routine use

4. What To whom Year 3 Deliverables Impact Many Jewels available, some already in routine use Demonstration breeding programs est. $60K saved from 2012 apple seedling culling alone!

5. More attention needed on translation to application (MAB Pipelining), less on QTL discovery Such translation is: - idiosyncratic to each breeding program - new to breeders and students Challenges and Plan for tackling  Solution: MAB Pipelining Taskforce Attention to each crop, each program represented Focus on: - marker conversion - allele effect calculations - allele distributions in germplasm - use in breeding

6. What To whom Planned Year 4 Deliverables Intended Impact More jewels! + the skills & knowledge to develop and use these and more Demonstration & Community Breeders Success stories & experiences useful for encouraging and guiding MAB for all U.S. Rosaceae breeders

7. To whom Breeders Planned Year 4 Deliverables Intended Impact More efficient development of new cultivars: (1)crosses yield more superior seedlings (2)early elimination of seedlings predicted to be genetically inferior What Breeder’s crossing and seedling selection decisions will be DNA-informed

8. OBJ. 5 EXTENSION Year 3 Deliverables, Challenges, Year 4 Goals

9. Goals Breeders: adoption of Marker-Assisted Breeding Allied Scientists: utilization of newly generated science based knowledge, tools and databases Industry: better understanding of the power of DNA- informed breeding leads to new ways to jointly accelerate commercialization and impact of new cultivars

10. What To whom Year 3 Deliverables Impact RosBREED Participatory Workshop, 30 July 2012: “Marker-Assisted Breeding in Action!” Community Breeders allied scientists Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; application of MAB in their breeding programs Request from breeders: more information on QTL and validation studies, especially within their programs

11. What To whom Year 3 Deliverables Impact Expansion of RosBREED network through presentations at meetings and informal gatherings – conducted Breeders, allied scientists, industry stakeholders Awareness and interest created at national and international meetings including “unveiling” of Jewels Fruit breeding working group sponsored RosBREED workshop at ASHS 2012 1. Plant & Animal Genome XX Conference, San Diego, CA, Jan 2012 - Presentations in Fruit & Nut Crops Workshop 2. 2012 FruitBreedomics First Annual Meeting, Prague, Czech Republic, Feb 2012 3. 2012 Biotechfruit 2012, Nelson, New Zealand, Mar 2012 4. 2012 American Society of Horticultural Science, Miaimi, FL, Aug 2012 5. 2012 Northwest Nursery Improvement Institute, Yakima, WA, Dec 2012

12. What To whom Year 3 Deliverables Impact Project-wide content – developed, disseminated and undertaken Project participants & stakeholders Stakeholders gain knowledge of project activities and deliverables Newsletter sent to 169 in 2012 (vs. 130 last year) RosBREED website visited by 3,168 (vs. 1,353 in 2011) unique visitors, from 87 (vs. 42 in 2011) countries (52% returning & 48% new visitors!) 94 recipients opened the Nov 2012 newsletter (vs. 79 last year) “RosBREED Jewels are now featured in eXtension!!!”

13. Obtain participation of a high proportion of Rosaceae Community Breeders at the “MAB in Action” Workshop: held workshop in conjunction with ASHS  Learning from the experience: Hold Crop-Specific MAB in Action Workshops prior to or immediately after major fruit meetings; generate interest in Workshops through publicizing success stories  Peach Workshop: was held just prior to a South Carolina Peach Council Meeting  Apple workshop: immediately before PAG meeting  Strawberry workshop: immediately after NASGA meeting Challenges and Plan for Tackling

14. What To whom Planned Year 4 Deliverables Impact Conduct RosBREED Crop-Specific MAB Workshops Peach - SC in Oct 2012; Apple - tomorrow in CA; Strawberry - January in OR Demonstration and Community breeders Plan spring crosses using knowledge of DNA information; Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; Application of MAB in their breeding programs Visit breeding programs on request to share RosBREED’s deliverables

15. What To whom Planned Year 4 Deliverables Intended Impact Continue to disseminate RosBREED deliverables and communicate with stakeholders through the use of published materials and participation in meetings Rosaceae breeders, allied scientists, industry Breeders: breed as efficiently, accurately, and creatively as possible Allied scientists: connect better with breeders; use newly generated science-based knowledge, tools, databases Industry: value & support DNA-informed breeding