1. Physical Pharmacy 21 Particle Size Sizing Technique 3: Laser Diffraction Photon Correlation Spectroscopy Kausar Ahmad Kulliyyah of Pharmacy, IIUM http://staff.iiu.edu.my/akausar

2. Physical Pharmacy 22 Light Scattering Reflected Diffracted Refracted When light strikes a particle, the light is then scattered in all direction.

3. Physical Pharmacy 23 Light scattered

4. Physical Pharmacy 24 Scattering Intensity

5. Physical Pharmacy 25 Static Light Scattering

6. Physical Pharmacy 26 Particle Sizing by Laser Diffraction

7. Physical Pharmacy 27 Mie Theory Application for particle size analysis

8. Physical Pharmacy 28 Laser Diffraction Configuration

9. Physical Pharmacy 29 Laser Diffraction Sizing Principle

10. Examples InstrumentLaser SourceDetectorLens Horiba LA-920He-Ne laser at 632.8 nm for large particles, tungsten lamp at 405 nm for small particles. Ring detector array of 75 high- purity silicon elements Coulter LS100Consists of 126 Malvern Mastersizer E 2 mW He-Ne laser at 632.8 nm 45 mm: 0.1 – 60  m 100 mm: 0.5 – 120  m 300 mm: 1.2 – 300  m The number of detectors is critical for high resolution Physical Pharmacy 210

11. Physical Pharmacy 211 Dynamic Light Scattering A.k.a. Photon Correlation Spectroscopy

12. Physical Pharmacy 212 DLS Principle

13. Physical Pharmacy 213 PCS setup

14. Physical Pharmacy 214 Characteristics of PCS/DLS

15. Physical Pharmacy 215 Laser Source for DLS

16. Physical Pharmacy 216 Factors influencing scattering intensity

17. 17Physical Pharmacy 2

18. 18Physical Pharmacy 2

19. 19Physical Pharmacy 2

20. 20Physical Pharmacy 2

21. 21 References http://www.photocor.com/photon_correlation_spectroscopy. htm SD Duke, RE Brown, EB Layendecker, “Calibration of spherical particles by light scattering”, Duke Scientific Corporation, Technical Note-002B, May 15, 2000.