1. Disk Stack Centrifuge Presented By: Gureet Chandhok Waqar Khan Blake Mc Cord CHEE 450: Engineering Biology

2. DISK STACK CENTRIFUGE A centrifuge is a piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis, applying force perpendicular to the axis. Essentially works like a clarifier providing better control over residence time.

4. Purpose of Centrifugation Since ours is a fermentation based bio process, the ISOLATION and the purification of the Insulin is the immediate goal once the fermentation step is concluded. In Insulin production, used to separate desired proteins from unwanted biomass.

5. Types Of Centrifuges Tricanter Disk Stack Centrifuge Decanter Sedicanter Belt Press Sorticanter

6. How does it Work? Disks are spaced out at 2mm. Spins rapidly exerting upto 12000 G force. Solid collects at the bottom liquids move up.

8. Operating Factors Solid holding space Viscosity Diameter of particle Difference in Density d 2 (ρ p - ρ 1 )g v t = ------------- 18μ Stokes Law!

9. Design Improvements Factors governing performance Volume of solids-Coagulation and Flocculation Settling Area-Residence Time

10. CH-36B GOF ® Manufacturer-Alfa Laval Type – Nozzle Centrifuge with continuous discharge Able to handle sensitive cell cultures Recirculation of solids Gentle gradual acceleration of product Minimization of shear forces preventing cell lysis No contact with oxygen No foaming or oxidation of product High grade stainless steel Steam sterilizable

11. Design Specifications Feed Rate-3888.81 kg/h Viscosity of Liquid-1.5 cP Height-3.85m G-4300 Speed- 2900 rpm Sedimentation Efficiency-30% Motor Power-26 kW Volume- 18.5m 3

12. Co$t Estimated to be ~$350,000-$650,000 Operating costs extra

13. References http://www.flottweg.de/Broschu/%DCbersichtsprospekt_E.pdf http://www.lsbu.ac.uk/biology/enztech/centrifugation.html#2_2 http://www.lenntech.com/Centrifugation.htm www.fao.org/docrep/003/x6899e/X6899E12.jpg http://www.alfalaval.com/digitalassets/2/file10080_0_PD41493e.pdf http://www.alfalaval.com/ecoreJava/WebObjects/ecoreJava.woa/wa/showN ode?siteNodeID=8720&contentID=25790&languageID=1

14. Questions?