1. Laboratory Animal Care BOSTON UNIVERISTY Animal Health Monitoring

2. Female Male

3. Estrus Detection in Mice Estrus Non-Estrus swollen, moist closed vaginal and open appearance opening of Vaginal opening.

4. Normal Females with Copulatory Plugs Copulatory plugs is made of several different components---sperm, ejaculate fluids, and mucus are primary substances that harden within the cervix.( Hard Waxy)

5. Healthy Mouse

6. Normal Rat

7. Normal Mouse Normal

8. Sick Mouse Hunched posture

9. Signs of Pain and Distress in Rodents Decreased activity Unkempt fur (not grooming) Pilo – erection Hunched posture Rapid shallow Breathing “ Red tears “ albino rats” Squinting of eyes Vocalization Feed and/or water refusal Weight loss

10. Alopecia Hair loss Due to excessive grooming Metabolic dysfunctions External parasites and Nutritional disorders

11. Facial Barbering Agouti (brown mouse) Dominant male chews off the hair from head, neck and back of the less dominant cage mate

12. Barbering vs Shaved Barbered Shaved

13. Ulcerative Dermatitis Syndrome Usually in black mice of a C57BL/6 background. Mice will develop a dermatitis with alopecia and skin ulcers. Mice will scratch at the effected region until ulcers form. The ulcerated areas often scar, causing restriction of limb movement. Any skin lesions should be brought to the attention of vet services.

14. Mild Dermatitis

15. Mild Dermatitis Early Detection is Important!

16. Dermatitis

17. More Severe Dematitis….

18. Too Much Caladryl !

19. Fight wounds

20. Fight Wounds Fighting is usually seen in males. The wounds are often seen on the tail, backs and genital area.

21. It is important to note fight wounds EARLY, Before they turn into….

22. Urine Scalding

23. Dystocia = difficult birth This is when a female has difficulty giving birth. Signs might include vaginal discharge, dehydration, and lethargy. Normal mice give birth only at night – if they are in labor during day, something is wrong. There may also be dead pups in the cage. Normal birth times = 1 – 3.5 hours for a litter of 11 pups

24. Vaginal Prolapse

25. Vaginal prolapse and ulcerative dermatitis

26. Rectal prolapse

27. Penile Prolapsed Alopecia!

28. Do you think this mass is too big? YES! It impedes movement

29. Mass in scrotal area

30. Mass with open lesion

31. Lesions on bottom surfaces of paws due to Burns

32. Would you say this mouse is pregnant? What if I told you that this mouse is a….

33. A MALE!!

34. Head Tilt

35. Irritated eye with discharge

36. Did you also note his irritated eye?

38. Severe Malocclusion

39. Trimming Malocclusion

40. Is there anything wrong with this mouse?

41. Hydrocephalus vs Normal

42. Ringtail (Tail Constriction)

43. Ringtail Possible cause Low Humidity

44. Is there a difference here?

45. Healthy vs. Sick Look at: 1.Eyes 2.Ears 3.Tail

46. Porphyrin in Rats (red tears) Porphyrin is naturally secreted, and normally groomed away and not seen in most instances.

47. Protruding eye = Exophthalmic

49. Rabbit Swollen foot

50. Step 1 Post Examine Cage sticker on Cage

51. Step 2 Fill out Request for Exam Form

52. Step 3 Vet Services will post Examine Cards on Cage after Assessment.

53. Animals Recovering from Surgery Blue cards NO Abbreviations for Procedures!!!

54. Your help in caring for all the animals at LASC is greatly appreciated!