1. Varying Alcohol Concentrations The Rats Drink Nasrin Akter Dr. Michael Lewis Hunter College Harlem Children Society

2. Introduction  Scientists use animals when the use of humans is either impractical or unethical.  For example when scientists investigate fetal alcohol syndrome, they cannot give alcohol to pregnant women but they can give to pregnant animals such as rats.  Scientific research with animals has contributed to many important advances in scientific & medical knowledge.

3. Intro Cont . Mice and rats are a useful model for studying the effects of alcohol.  The SD rats are more active during the night then during the day.  SD rat is a general model for the study of human health and disease.  The SD rats is a widely accepted, dependable, general purpose research model used in virtually all disciplines of biomedical research including toxicology and pharmacology.

4. http://www.hku.hk/launit/pictures/sc_rat.jpg

5. Materials  Sprague Dawley (SD) rats  1% concentration=10 ml EtOH, 990 ml H2O  3% concentration=30 ml EtOH, 970 ml H2O  5% concentration=50 ml EtOH, 950 ml H2O  7% concentration= 70 ml EtOH, 920 ml H2O  9% concentration=100 ml EtOH,910 ml H2O

6. ithttp://www-personal.umich.edu/~nbutler/ninab7.jpghttp://www-personal.umich.edu/~nbutler/ninab7.jpg

7. Methods  We feed EtOH to SD rats once a week. We weight the bottles into grams (g) before we feed them and also after we feed them.  Record the time we place the bottles on the cage.  Wait exactly one hour before we remove the bottles.  Each rat has its own separate EtOH and H2O bottle.  There is 22 SD rats, Each bottle is numbered 1-22 for rat 1-22 there was sign that was set up for the bottle.  Bottles with orange tape are for EtOh and those with blue are for water (rats#1 gets orange#1 and blue #1)

8. Que$tion  Do SD rats drink more alcohol (EtOH) than water (H 2 0), or more water (H 2 0) than alcohol (EtOH)?

9. Average Alcohol and Water the Rats Drank 0 1 2 3 4 5 6 0246810 Concentration (%) Amount (g) Alcohol Water

10. References  http://www. personal.umich.edu/~nbutler/ninab7.jpg http://www. personal.umich.edu/~nbutler/ninab7.jpg  http://www.hku.hk/launit/pictures/sc_rat.jpg http://www.hku.hk/launit/pictures/sc_rat.jpg  http://science.education.nih.gov/supplements/ni h3/alcohol/guide/info-alcohol.htm http://science.education.nih.gov/supplements/ni h3/alcohol/guide/info-alcohol.htm  http://www.liq.wa.gov/PriceBook/bac_page.asp http://www.liq.wa.gov/PriceBook/bac_page.asp  http://www.franklin.uga.edu/casar/Training/rat %20training/Biomethodology%20of%20the%2 0Rat.htm#Behavior http://www.franklin.uga.edu/casar/Training/rat %20training/Biomethodology%20of%20the%2 0Rat.htm#Behavior

11. Acknowledgments  Dr. Michael Lewis  Dr. Sat Bhattacharya  Micki  Daisy  Harlem children society  Hunter College  M.S.K.C.C