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Published byMark Alderman Modified over 9 years ago
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Experiment Seven: Spike Referencing
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Voltage is the difference in electrical potential between two points (like your hair and the comb)….
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and like the difference between electrical environment around recording electrode and reference electrode
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Differential recordings: voltage = difference between recording and ground electrode. If “perfect ground”, voltage = potential at recording electrode. However…
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In biology, there is no such thing as the perfect ground! http://tinyurl.com/7qcz5kshttp://tinyurl.com/73lwcbu Everywhere in your body there’s an electrical signal of some sort, so care must be taken choosing your ground to minimize this.
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Procedure: put recording electrode in femur and ground electrode in coxa When you touch the barbs on the leg, make note of the level of “background activity” vs. individual spikes.
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Procedure: Move ground electrode from coxa to the femur When you touch the barbs on the leg, is there a difference in the ratio of “background activity” vs. individual spikes?
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Procedure: Move both electrodes to coxa What do you notice this time?
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