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Published byAlayna Bick Modified over 9 years ago
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Enzymes that attack “N” substrates 1. Urease 2. Gelatinase 3. Nitrate Reductase
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Urease Urea + water ammonia + carbon dioxide Urea Broth Urea pH Indicator Acidic = Yellow Basic = Pink
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Gram Reaction Morphology O2Ferment Lactose Urease E. coli (-) Bacilli Facultative (+) (-) Proteus vulgaris (-) Bacilli Facultative (-) (+) Salmonella (-) Bacilli Facultative (-) Shigella (-) Bacilli Facultative (-)
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Urease Test
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Gelatinase Gelatin (solid) Gelatin (liquid) Tryptic Soy Gelatin Agar
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Gelatinase Test
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Nitrate Reductase Nitrate Nitrite + water Facultative Bacteria Nitrate Broth
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