1. www.wilksIR.com InfraCal TOG/TPH Analyzer, Model CVH Extraction Procedure

2. The sample extraction can be done using either a prescription bottle with a septa cap or a separatory funnel

3. Extraction using a prescription bottle with a septa cap

4. Collect a measured amount of sample Collect a measured amount of sample

5. For samples that will be stored for more than a day or for samples with a high pH: Adjust the pH to less than 2 with Hydrochloric acid or Sulfuric Acid (typically 3-5 drops depending on buffers in sample)

6. Add Solvent Add Solvent 10 parts sample to 1 part solvent For example: For 150 ml of sample add 15 ml of solvent

7. Shake for 2 minutes Shake for 2 minutes

8. Open the cap on the bottle during the 2 minute shaking to relieve the pressure Open the cap on the bottle during the 2 minute shaking to relieve the pressure

9. Solvent is heavier than water and will sink

10. Turn bottle upside down Turn bottle upside down

11. Use the syringe to remove the solvent Use the syringe to remove the solvent

12. Fill the cuvette with solvent Fill the cuvette with solvent

13. Place the cuvette in the InfraCal frosted side to the front Place the cuvette in the InfraCal frosted side to the front

14. Press the run button Press the run button

15. Oil concentration displayed Oil concentration displayed 14

16. Extraction using a separatory funnel

17. Pour measured sample into separatory funnel Pour measured sample into separatory funnel

18. Measure one tenth of the sample size of solvent Measure one tenth of the sample size of solvent For example: For 150 ml of sample add 15 ml of solvent

19. Pour the solvent into the sample collection container to rinse interior surfaces and cap of left over oil Pour the solvent into the sample collection container to rinse interior surfaces and cap of left over oil

20. Pour the solvent from the sample container into separatory funnel containing sample Pour the solvent from the sample container into separatory funnel containing sample

21. Shake for 2 minutes Shake for 2 minutes

22. Stop to vent during the 2 minutes shaking to relieve the pressure Stop to vent during the 2 minutes shaking to relieve the pressure

23. Put the separatory funnel into the stand Put the separatory funnel into the stand

24. Allow the solvent to sink to the bottom Allow the solvent to sink to the bottom

25. Fill the cuvette with solvent Fill the cuvette with solvent

26. Place the cuvette in the InfraCal frosted side to the front Place the cuvette in the InfraCal frosted side to the front

27. Press the run button Press the run button

28. Oil concentration displayed Oil concentration displayed 14

29. Silica Gel Extraction The difference between TPH (Total Petroleum Hydrocarbon) and TOG (Total Oil and Grease) is the polar organics are removed from the extract using silica gel. The remaining hydrocarbons are the non-polar components considered to be TPH

30. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica

31. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

32. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel

33. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Put solvent extract into cuvette Put solvent extract into cuvette

34. Silica Gel Extraction Put filter paper into funnel Put filter paper into funnel Add silica Add silica Put funnel into bottle Put funnel into bottle Put solvent extract through Put solvent extract through silica gel Put solvent extract into cuvette Put solvent extract into cuvette Put cuvette into InfraCal frosted side to the front Put cuvette into InfraCal frosted side to the front

35. Press the run button Press the run button

36. Oil concentration displayed Oil concentration displayed 14

37. For questions please contact us at: tech@WilksIR.com, or 203-855-9136 InfraCal TOG/TPH Analyzer, Model CVH Extraction Procedure