1. The Effects of Pollution on Bioluminescent Bacteria By: Tobi Simon

2. Need Long-term exposure Long-term exposure Plants and Animals Plants and Animals http://www.peternjenga.com/blogs/greenkenya/wp-content/uploads/2009/03/natural-and-manufactured-air-pollution.png

3. Knowledge Base http://nptel.iitm.ac.in/courses/Webcourse-contents/IIT- Delhi/Environmental%20Air%20Pollution/air%20pollution%20(Civil)/Module-1/images1/35.gif

4. Knowledge Base http://www.mcbs.unh.edu/faculty/Whistler/images/image3.jpg Bioluminescent Bacteria

5. Literature Review Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006). Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006).

6. Literature Review Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008). Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008).

7. Literature Review Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988). Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).

8. Literature Review Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005). Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005).

9. Purpose The effects of pollution on the amount of light emitted from bioluminescent bacteria.

10. Hypothesis Alternate: Water with higher amounts of hydrocarbons will cause a decrease in the amount of light emitted from the bacteria Null: Water with hydrocarbons in it will have no effect on the amount of light emitted from the bacteria.

11. Methodology Water is collected from different locations and a sample of each is tested for the amount of hydrocarbons present. Bioluminescent bacteria are placed into different samples of water After 24 hours the BLR will be calculated for each sample of water. Control No hydrocarbon in water Variable 1 Lowest amount of hydrocarbon in water Variable 2 Medium amount of hydrocarbon in water Variable 3 Highest amount of hydrocarbon in water Statistical Analysis: ANOVA followed by a Sheffe post hoc test

12. Do Ability Organisms Organisms Procedures Procedures http://www.abdn.ac.uk/staffpages/uploads/bch052/aglover-bac.jpg

13. Budget VendorOrder #QuantityDescriptionPrice Total Price SigmaF7911-100EA1Millipore glass-fiber filters86.7 SigmaA7002-250G1Agar63 SigmaPeptone SigmaS9148-1L10Seawater20.7207 SigmaY1626-250G1Yeast Extract115 Incubator Spectrophotometer SigmaP5481-500EA1Petri dishes83.1 Carolina ScienceNP-15-57233Vibrio fischeri10.9532.85

14. Bibliography American College of Gastroenterology. “Air Pollution May Increase Risk Of Appendicitis.” Science Daily 7 October 2008. 23 April 2009 American College of Gastroenterology. “Air Pollution May Increase Risk Of Appendicitis.” Science Daily 7 October 2008. 23 April 2009 Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008). Alvarez, David A. "Chemical and Toxicologic Assessment of Organic Contaminants in Surface Water Using Passive Samples." Journal of Environmental Quality (2008). Bernstein, Leonard. Environmental Science Ecology and Human Impact. 2 nd ed. Menlo Park, California: Addison-Wesley Company, 1996. Bernstein, Leonard. Environmental Science Ecology and Human Impact. 2 nd ed. Menlo Park, California: Addison-Wesley Company, 1996. "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007. "Bioluminescence." McGraw-Hill Encyclopedia of Science and Technology. 10th ed. 2007. Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006). Bolster, Carl H. "Comparison of E. coli and C. jejuni Transport in Saturated Porous Media." Journal of Environmental Quality (2006). Bullock, Carole. “Air pollution may cause and speed up artery disease.” EurekAlert! Bullock, Carole. “Air pollution may cause and speed up artery disease.” EurekAlert! Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005). Frischer, M. E. "Bioluminescent Bacteria as Indicators of Chemical Contamination of Coastal Waters." Journal of Environmental Quality (2005). Gulifoy, Christine. “Inhaling a heart attack: How air pollution can cause heart disease.” EurekAlert! Gulifoy, Christine. “Inhaling a heart attack: How air pollution can cause heart disease.” EurekAlert! International Union of Forest Research Organizations. “Damage To Forests From Climate Change Could Cost The Planet Its Major Keeper Of Greenhouse Gases, Study Warnes.” ScienceDaily. 21 April 2009. International Union of Forest Research Organizations. “Damage To Forests From Climate Change Could Cost The Planet Its Major Keeper Of Greenhouse Gases, Study Warnes.” ScienceDaily. 21 April 2009. Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed. Safra, Jacob E. "Bioluminescence." The New Encyclopedia Britannica. 15th ed. University of Arizona. “Fire Influences Global Warming More Than Previously Thought.” ScienceDaily 29 April 2009. University of Arizona. “Fire Influences Global Warming More Than Previously Thought.” ScienceDaily 29 April 2009. http://www.sciencedaily.com/releases/2009/04/090423142332.htm. http://www.sciencedaily.com/releases/2009/04/090423142332.htm. http://www.sciencedaily.com/releases/2009/04/090423142332.htm University of Oxford. “Ice Sheet Behavior Much More Volatile And Dynamic Than Previously Thought, Tahiti Corals Show.” ScienceDaily 30 April 2009. 29 April 2009 University of Oxford. “Ice Sheet Behavior Much More Volatile And Dynamic Than Previously Thought, Tahiti Corals Show.” ScienceDaily 30 April 2009. 29 April 2009 Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988). Vanhaecke, Erwin. "Bioluminescence Assay for Measuring the Number of Bacteria Adhering to the Hydrocarbon Phase in the BATH Test." Applied and Environmental Microbiology (1988).