Download presentation
Published byMoris Stone Modified over 9 years ago
1
MNTP Summer Workshop fMRI BOLD Response to Median Nerve Stimulation: A Comparison of Block and Event-Related Design Mark Wheeler Destiny Miller Carly Demopoulos Kyle Dunovan Martin Krönke Todd Monroe Dil Singhabahu Elisa Torres Christopher Walker Funded by: NIH R90DA02342
2
MNTP Workshop: Learning Objectives
In-depth understanding of preprocessing of fMRI data Filtering Motion correction Slice Time Correction Smoothing Registration Conduct first-level analyses Conduct group-level analyses Investigate two experimental designs
3
The Task: Median Nerve Stimulation
Electrical stimulation of the median nerve by applying pulses to the wrist of the non-dominant hand Voltage: motor threshold
4
Blocked Design 10 repetitions Pros. Cons.
Stim ON 10s Stim OFF 16s Stim ON 10s Stim ON 10s 15Hz 15Hz 15Hz Stim OFF 16s Stim OFF 16s 10 repetitions Pros. Cons. Excellent detection power (knowing which voxels are active) Useful for examining state changes Poor estimation power (knowing the time course of an active voxel) Relatively insensitive to the shape of the hemodynamic response.
5
Event-Related Design Pros. Cons.
Good at estimating shape of hemodynamic response Provides good estimation power (knowing the time course of an active voxel) Can have reduced detection power (knowing which voxels are active) Sensitive to errors in predicted hemodynamic response
6
Event Related Task Design
Three different frequencies: 15Hz, 40Hz, 80Hz (Kampe, Jones & Auer, 2000) Event length: 4s Inter-stimulus jitter – 2, 4, 6 seconds Exponential distribution (Dale, 1999) 15Hz + 40Hz 80Hz 4s (2TR) 4s Time 2s Jitter 6s Jitter 4s Jitter
7
Data Acquisition Scanner: Allegra 3T N=5 Structural Scan
T1 weighted MPRAGE 176 slices Voxel Size 1mm Functional Scans: Median Nerve Stimulation Volumes 140 for block 233 for event-related Voxel Size 3.5mm Slices 34 Interleaved TR 2s T2* contrast
8
Single Subject Demonstration
Processing stream Data-conversion Dicom2Nifti Statistical analysis GLM Statistical Parametric Mapping Preprocessing Block Design Single Subject Demonstration Slice-timing Motion correction Temporal Filtering Smoothing Registration / Normalization
9
Preprocessing: Slice Time Correction (STC)
Huettel, Song, McCarthy 2009 Stronger influence of STC for event-related vs. block-designs sensitivity to timing / shape of HRF Slice acquisition order interleaved slice acquisition (34 slices in 2s) avoids cross-slice excitation Debate on STC before / after motion correction? before head motion (interleaved) Temporal non-linear sinc interpolation
10
Motion correction Due to subject movements inside the scanner, a voxel might represent different parts of the brain across time points, introducing artifacts Huettel, Song, McCarthy, 2004
11
Motion correction Estimation Rigid-body transformation 6 DOF 0.2 mm
-0.1 time (TRs) radians 0.003 -0.004 time (TRs) Interpolation trilinear Nearest neighbour (tri-)linear Non-linear (sinc, B-spline)
12
No Motion correction Motion corrected Z-Value: 3.9
% signal change Z-Value: 3.9 Crosshair location: Postcentral gyrus Time (TRs) Motion corrected % signal change Time (TRs) Z-Value: 3.8
13
Temporal Filtering A highpass filter can remove these unwanted effects
Artifacts like “slow scanner drift” and changes in basal metabolism can reduce SNR A highpass filter can remove these unwanted effects Do not want to remove task-related signal Block Design Task: 10s on, 16s rest Woolrich et al. (2001) recommends filter of at least 2 epochs duration 52s temporal filter .019 Hz Also compared effects of 0 Hz, .038 Hz, .01 Hz Little difference between .019 Hz .038 Hz .01 Hz Discrete cosine transform Low frequencies 0 – 0.1 Hz 0.1 – 0.5 Hz respiratory Hz cardiac
14
0Hz / No Temporal Filtering
% Signal Change Time (TRs) 52s / .019Hz Temporal Filter % Signal Change Time (TRs)
15
Smoothing Spatially filters data using Gaussian Kernel to remove noise
Reduces spatial resolution Improves signal to noise ratio Consider ROI and voxel size in determining the size of the kernel Gaussian Weight
16
0mm smooth 4mm smooth 8mm smooth 20mm smooth
17
Registration / Normalization
Why? Group analysis Compare results in common coordinate system (MNI) Karsten Müller How? Estimate transformation Combining affine-linear (12 DOF) subject standard space (FSL FLIRT) nonlinear methods (> 12 DOF) subject subject (FSL FNIRT) least squares cost function 2. Resample / Transform / Interpolate Nearest neighbour Linear interpolations Bi-, trilinear Non-linear interpolations B-Spline, sinc (Hanning)
18
Preprocessing Summary
Data-conversion Dicom2Nifti Block Design Filtering Highpass (52s / .019Hz) Discrete cosine transform Motion correction Rigid-body, 6DOF Trilinear interpolation Statistical analysis GLM 1st-level Group-analyses Slice-timing Interleaved Sinc interpolation Smoothing FWHM, 8mm Registration / Normalization Affine-linear + Non-linear Statistical Parametric Mapping
19
Design matrix comparison: Block vs. Event-related
Block design 15Hz Time Event-related 40Hz 80Hz 15Hz
20
Block vs. Event-Related Design
Block Design 15Hz activation map Modeled with gamma function Event-Related Design 15Hz activation map Modeled with double-gamma function
21
Functionally vs.structurally defined ROIs
ROI (structure) ROI (functional 9 mm) ROI (functional 6 mm) ROI (functional 3 mm)
22
Effect of Region of Interest on Task Related Median Percent Signal Change
-0.10 0.00 0.10 0.20 0.30 0.40 0.50 15Hz 40Hz 80Hz 80Hz > All* Functionally Defined Structurally Defined Median Percent Signal Change Median percent signal change of the BOLD response to 15, 40, and 80 Hz median nerve stimulation from baseline. The functionally defined ROI was defined as above; the structurally determined ROI was created using the Harvard-Oxford Structural Atlas defined boundaries for the postcentral gyrus, and masked to include only right hemisphere activity. *Note: “80 Hz > All” denotes the contrast of the response to 80 Hz stimulation against both the 15 and 40 Hz responses combined. It has been included to demonstrate the potential to model differences between conditions with the GLM approach. ROI – F (1, 4) = 6.431, p = .064 Frequency – F (2, 4) = , p = .007 Frequency * ROI – F (2, 8) = 5.101, p = .037
23
Future Directions: Condition and Subject Timeseries
Modeled 15 Hz response for 1 subject Arbitrary Units
24
Event-Related Activation Comparison
15 Hz above baseline 40 Hz above baseline 80 Hz above baseline
25
Future Directions: Overlapping Activation
Investigate condition specific differences in activation patterns
26
References Dale, A. M. (1999). Optimal experimental design for event-related fMRI. Human Brain Mapping, 8: 109–114.doi: /(SICI) (1999)8:2/3<109::AID- HBM7>3.0.CO;2-W Huettel, S. A., Song, A. W. and McCarthy, G. (2004). Functional magnetic resonance imaging. Sunderland, MA: Sinauer Associates Kampe, K. K., Jones, R. A. and Auer, D. P. (2000). Frequency dependence of the functional MRI response after electrical median nerve stimulation. Human Brain Mapping, 9: 106–114. doi: /(SICI) (200002)9:2<106::AID- HBM5>3.0.CO;2-Y Woolrich, M. W., Ripley, B. D., Brady, M., Smith, S. M. (2001). Temporal autocorrelation in univariate linear modeling of FMRI data. NeuroImage, 14,
27
Thank you Mark Wheeler Destiny Miller Seong-Gi Kim Bill Eddy
Tomika Cohen Rebecca Clark Fellow MNTPers! Funded by: NIH R90DA02342 27
Similar presentations
© 2025 SlidePlayer.com. Inc.
All rights reserved.