1. Pfam Pfam & DAS Rob Finn 26 th Feb 2007

2. Pfam Acknowledgements John Tate Roger Pettett Andreas Prlic Andy Jenkinson But takes data from community…..!

3. Pfam New Pfam Website!

4. Pfam New Pfam Website! DAS Enabled

5. Pfam What do we provide? Pfam Domains Active Sites Other sequence Features Alignments Alignment features

6. Pfam Pfam Alignment Client Motivation –Simple alignment client Not trying to reinvent Jalview –Markup alignment –Pfam alignments vary in size Paging

7. Pfam Pfam Alignment Client Example - combines alignment and features requests

8. Pfam Pfam Features Client Motivation –Include Other annotations Identify where we are missing domains –Reduce data duplication –Allow tailored views –Enrich single protein data in Pfam

9. Pfam Pfam Features Client

10. Pfam Other Datasets

11. Pfam What Code Base? Pfam is Perl orientated –Server ProServer (CPAN) –Client Bio::Das::Lite (CPAN) Bio::Pfam::Drawing –Break out?

12. Pfam What Can’t I Do (with Das)? Asynchronous requests –Return pfam_scan results via DAS Mapping experimental datasets to public datasets Serve Pfam Textual Annotation –SOAP implementation

13. Pfam What is Hard to Do? Large scale analyses Filter out rubbish! –But what is one man’s rubbish…. Requirement for stylesheet? Standard Graphics library?

14. Pfam What Worries Me? Data deluge –Make Views….? Other people serve Pfam data –Confusion…..? –Redundancy People always want more from clients

15. Pfam Thank you!