1. Prince Henry’s Institute Monash Medical Centre 246 Clayton Road, Clayton, Victoria Contacts: Dr. Morag Young: morag.young@princehenrys.orgmorag.young@princehenrys.org Prof. Peter Fuller: peter.fuller@princehenrys.orgpeter.fuller@princehenrys.org Steroid Receptor Biology

2. Mineralocorticoid receptors (MR) and Heart Disease Heart disease is a leading cause of death in Australia (40%) MR are a key players in this pathology…but how?! Dr Morag Young Uninephrectomised mouse/rat treated with aldosterone/DOC plus 0.9% saline Cardiac FibrosisHypertension Cardiac Hypertrophy Oxidative Stress NADPH oxidase Inflammation COX-2 Osteopontin Macrophages 8 days 8 weeks We have 3 tissue selective MR knockout mouse strains that we are using to determine the specific role of the MR in heart

3. Mineralocorticoid receptors (MR) and Heart Disease Dr Morag Young Fibrillar collagen Fibroblast VSMC Endothelial cell Cardiac myocyte Artery Macrophage MR-null mice: No effect on DOC-induced monocyte/macrophage recruitment Reduced basal gene expression Cardiac myocyte MR-null mice: No effect on DOC-induced monocyte/macrophage recruitment No effect on inflammatory gene expression Endothelial cell MR-null mice: Reduced DOC-induced macrophage recruitment at 8 days MR

4. Granulosa Cell Tumours (GCT) Comprise 5% of malignant ovarian tumours Molecular analysis of tumour bank and cell lines Tissue selective knockout of IKK signalling in ovaries Our focus has been on: profiles of gene expression: candidates & microarray signalling pathways – constitutive activation expression of ER  coregulatory molecules ERβ-induced genes mutation detection Implications for normal granulosa cells Professor Peter Fuller