1. DH110 Oral Histology and Embryology Lesson 3 Tammy R. Fisher RDH BS Teeth Development, Eruption and Shedding

2. Roll Call T R Achievement- Tell us one of yours! C I

3. Objectives for Lesson 3 Review of Chapters 1-4 Quiz #1

4. Break 1 Grade Homework #1

5. Odontogenesis Stages of Tooth Development 1. Bud Stage 2. Cap Stage 3. Bell Stage 4. Dentinogenesis

57. Group Activities Group 1 Consider the Patient Chapter 5&6 Group 2 Self Evaluation Questions Chapter 5 Group 3 Self Evaluation Questions Chapter 6

58. Group Discussions Group 1 Radiographs and Consider the Patient Group 2 Self Evaluation Questions Chapter 5 Group 3 Self Evaluation Questions Chapter 6

59. Bio-engineered Teeth Volponi, A., Kawasaki, M., & Sharpe, P. (2013). Adult human gingival epithelial cells as a source for whole tooth bio-engineering. Journal of Dental Research, 92(4), 329-334. http://www.cbsnews.com/video/watch/?id=50142931n http://www.cbsnews.com/video/watch/?id=50142931n Teeth develop from interactions between embryonic oral epithelium and neural-crest-derived mesenchyme. These cells can be separated into single- cell populations and recombined to form normal teeth, providing a basis for bioengineering new teeth if suitable, non-embryonic cell sources can be identified. We show here that cells can be isolated from adult human gingival tissue that can be expanded in vitro and, when combined with mouse embryonic tooth mesenchyme cells, form teeth. Teeth with developing roots can be produced from this cell combination following transplantation into renal capsules. These bioengineered teeth contain dentin and enamel with ameloblast-like cells and rests of Malassez of human origin.

60. Summary https://www.youtube.com/watch?v=5kRTtTYhtCU

61. Discuss Next Week Read Chapter 7 and 8. You may want to turn in Homework #2 next week.