1. Prostate: Transverse section

2. Radical Prostatectomy
Bladder neck
Apex

3. Radical Prostatectomy
Base/
Bladder Neck
Apex
Posterior quadrants of alternate slices have been removed and frozen after inking the surface

4. Fresh, non-frozen tissue
Lateral aspect
Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data
Seminal
Vesicle
Permanent section
Permanent section
right
left 1 2 6 7 3 4 5 5R 5
5R 5L 5L
Fresh, non-frozen tissue
for flow sorting
Frozen section
Unfixed tissue
for RNA & DNA
right
left 4R
Frozen section 4
4R 4L 4L
Permanent section
right
left
permanent section 3R 3
3R 5L 3L
= Prostate carcinoma

5. Fresh, non-frozen tissue: cell sorting
Human prostate (lateral view)
Obtaining samples of non-tumor tissue from a radical prostatectomy specimen.
Seminal
Vesicle
Permanent
section
Fixed tissue:
In-situ hybridization 1 2 6 7
right
left 3 4 5 5R 5
5R 5L 5L
Fresh, non-frozen tissue: cell sorting
This block is snap frozen (isopentane in liquid N2), within < 20 minutes of devitalization
Frozen tissue:
Immunostains
Laser capture
right
left 4R 4
4R 4L 4L
Frozen section
Permanent
section
right
left
Permanent
section 3R 3
3R 5L 3L
= Prostate carcinoma

6. Prostate (transverse section)

7. Normal prostate parenchyma (high magnification)
Basal epithelial cells
Secretory (luminal) epithelial cells
Fibromuscular stromal cells

8. Normal prostate parenchyma (low magnification)
fibromuscular stroma
normal glands
lymphocytes