1. Elizabeth M. Whitley, DVM, PhD, Dipl. ACVP 1 Isabel Hurtado Rodriguez, DVM, MS, Dipl. ACVIM 2 Amelia S. Munsterman, DVM, MS, Dipl. ACVS 2 1 Department of Pathobiology 2 Department of Clinical Sciences College of Veterinary Medicine Auburn University www.sogonow.com/archives/2007/01/07-week/ Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

2.  5-year-old, male castrated, American quarter horse  Used for roping  Recent, intermittent reluctance to work on the right hand  Recent, unexplained weight loss www.sogonow.com/archives/2007/01/07-week/ Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

3.  Presented for colic that was non-responsive to Banamine and sedation  Clinical signs:  Dehydration, tachycardia, tachypnea  Copious nasogastric reflux when tubed  Distended small intestine on ultrasound and palpation  Diagnosed a renal mass on ultrasound Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

4. Nephroblastoma  Rare neoplasm in animals other than pigs or chickens  Usually observed in young animals  “Wilms’ tumor” in children http://www.nlm.nih.gov/medlineplus/ency/imagepages/90 56.htm Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

5. Nephroblastoma  Three different morphologies  Blastemal (undifferentiated, embryonic) cells  Epithelial cells  Mesenchymal cells  Benign or malignant en.wikibooks.org/.../Pregnancy_and_birth Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

10. VimentinDesmin Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

11. Smooth muscle actin Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

12. Cytokeratin Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

13. Basement membrane Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

14.  Malignant nephroblastoma, triphasic, kidney, with metastasis to liver, spleen, and mesentery. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

15.  Inactivation of the Wilms’ tumor gene (WT1)  WT1 protein is a transcription factor that functions as a tumor suppressor gene ▪ Is expressed during renal and gonadal development  Defects in WT1 gene prevents normal differentiation of metanephric stem cells  Other genetic defects identified in children with Wilms’ tumor ( including WT2, WT3, WT4) Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

16.  Thanks to Dr. Eva Sartin, Beth Landreth, Uschi Wolfert for technical assistance in histopathology. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only