2. 2008-2009 gills alveoli elephant seals Gas Exchange Respiratory Systems

4.  Need O 2 in  for aerobic cellular respiration  make ATP  Need CO 2 out  waste product from Krebs cycle O2O2 food ATP CO 2 respiration for respiration

5.  O 2 & CO 2 exchange between environment & cells  need moist membrane  need high surface area

6.  Why high surface area?  maximizing rate of gas exchange  CO 2 & O 2 move across cell membrane by diffusion ▪ rate of diffusion proportional to surface area  Why moist membranes?  moisture maintains cell membrane structure  gases diffuse only dissolved in water High surface area? High surface area! Where have we heard that before?

7. one-celledamphibiansechinoderms insectsfishmammals endotherm vs. ectotherm size cilia water vs. land

8. Aquatic organisms external systems with lots of surface area exposed to aquatic environment moist internal respiratory tissues with lots of surface area Terrestrial

10.  Water carrying gas flows in one direction, blood flows in opposite direction just keep swimming…. Why does it work counter current? Adaptation!

11.  Blood & water flow in opposite directions  maintains diffusion gradient over whole length of gill capillary  maximizing O 2 transfer from water to blood water blood front back blood 100% 15% 5% 90% 70%40% 60%30% 100% 5% 50% 70% 30% water counter- current concurrent

12.  Advantages of terrestrial life  air has many advantages over water ▪ higher concentration of O 2 ▪ O 2 & CO 2 diffuse much faster through air ▪ respiratory surfaces exposed to air do not have to be ventilated as thoroughly as gills ▪ air is much lighter than water & therefore much easier to pump ▪ expend less energy moving air in & out  Disadvantages  keeping large respiratory surface moist causes high water loss ▪ reduce water loss by keeping lungs internal Why don’t land animals use gills?

13.  air tubes branching throughout body  gas exchanged by diffusion across moist cells lining terminal ends, not through open circulatory system Tracheae

14. Exchange tissue: spongy texture, honeycombed with moist epithelium Why is this exchange with the environment RISKY?

15.  Gas exchange across thin epithelium of millions of alveoli  total surface area in humans ~100 m 2

16.  Breathing due to changing pressures in lungs  air flows from higher pressure to lower pressure  pulling air instead of pushing it

17.  Air enters nostrils  filtered by hairs, warmed & humidified  sampled for odors  Pharynx  glottis  larynx (vocal cords)  trachea (windpipe)  bronchi  bronchioles  air sacs (alveoli)  Epithelial lining covered by cilia & thin film of mucus  mucus traps dust, pollen, particulates  beating cilia move mucus upward to pharynx, where it is swallowed

18. don’t want to have to think to breathe!  Medulla sets rhythm & pons moderates it  coordinate respiratory, cardiovascular systems & metabolic demands  Nerve sensors in walls of aorta & carotid arteries in neck detect O 2 & CO 2 in blood

19.  Monitors CO 2 level of blood  measures pH of blood & cerebrospinal fluid bathing brain ▪ CO 2 + H 2 O  H 2 CO 3 (carbonic acid) ▪ if pH decreases then increase depth & rate of breathing & excess CO 2 is eliminated in exhaled air

20.  Homeostasis  keeping the internal environment of the body balanced  need to balance O 2 in and CO 2 out  need to balance energy (ATP) production  Exercise  breathe faster ▪ need more ATP ▪ bring in more O 2 & remove more CO 2  Disease  poor lung or heart function = breathe faster ▪ need to work harder to bring in O 2 & remove CO 2 O2O2 ATP CO 2

21.  Concentration gradient & pressure drives movement of gases into & out of blood at both lungs & body tissue bloodlungs CO 2 O2O2 O2O2 bloodbody CO 2 O2O2 O2O2 capillaries in lungscapillaries in muscle

22.  Why use a carrier molecule?  O 2 not soluble enough in H 2 O for animal needs ▪ blood alone could not provide enough O 2 to animal cells ▪ hemocyanin in insects = copper (bluish/greenish) ▪ hemoglobin in vertebrates = iron (reddish)  Reversibly binds O 2  loading O 2 at lungs or gills & unloading at cells cooperativity heme group

23.  Binding O 2  binding of O 2 to 1 st subunit causes shape change to other subunits ▪ conformational change  increasing attraction to O 2  Releasing O 2  when 1 st subunit releases O 2, causes shape change to other subunits ▪ conformational change  lowers attraction to O 2

24. Bohr Shift  drop in pH lowers affinity of Hb for O 2  active tissue (producing CO 2 ) lowers blood pH & induces Hb to release more O 2 P O 2 (mm Hg) 0 10 20 30 40 50 60 70 80 90 100 020406080100120140 More O 2 delivered to tissues pH 7.60 pH 7.20 pH 7.40 % oxyhemoglobin saturation Effect of pH (CO 2 concentration)

25. Bohr Shift  increase in temperature lowers affinity of Hb for O 2  active muscle produces heat P O 2 (mm Hg) 0 10 20 30 40 50 60 70 80 90 100 020406080100120140 More O 2 delivered to tissues 20°C 43°C 37°C % oxyhemoglobin saturation Effect of Temperature

26.  Dissolved in blood plasma as bicarbonate ion Tissue cells Plasma CO 2 dissolves in plasma CO 2 combines with Hb CO 2 + H 2 OH 2 CO 3 H+ + HCO 3 – HCO 3 – H 2 CO 3 CO 2 Carbonic anhydrase Cl– carbonic acid CO 2 + H 2 O  H 2 CO 3 bicarbonate H 2 CO 3  H + + HCO 3 – carbonic anhydrase

27.  Lower CO 2 pressure at lungs allows CO 2 to diffuse out of blood into lungs Plasma Lungs: Alveoli CO 2 dissolved in plasma HCO 3 – Cl – CO 2 H 2 CO 3 Hemoglobin + CO 2 CO 2 + H 2 O HCO 3 – + H +

28.  Mother & fetus exchange O 2 & CO 2 across placental tissue Why would mother’s Hb give up its O 2 to baby’s Hb?

29.  HbF has greater attraction to O 2 than Hb  low % O 2 by time blood reaches placenta  fetal Hb must be able to bind O 2 with greater attraction than maternal Hb What is the adaptive advantage? 2 alpha & 2 gamma units

30. 2008-2009 Don’t be such a baby… Ask Questions!!