1. Biological Molecules Lipids ProteinsNucleic Acids Carbohydrates

2. Hydroxyl ( -OH ) Carbonyl (C=O) OHCO Alcohols Aldehydes, Ketones

5. USE A WATER BATH

6. Plant starch turn to a blue-black color; Glycogen (animal starch) turns red-brown; Cellulose (the major component of plant cell walls) turns violet-brown to red-brown. Lugol’s Iodine Liver cells Plant cells Potato

7. Lipids

8. Sudan III

9. Proteins

10. Biuret Reagent The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and sodium hydroxide (NaOH). The NaOH is there to raise the pH of the solution...

11. Nucleic Acids

12. Qiagen DNAeasy kit. Quantify our yields using a Nanodrop system.

13. Controls:  water  1% glucose (a reducing sugar)  1% egg albumin (a protein)  1% soluble plant starch (a polysaccharide)  corn oil (a lipid) Procedures and Assignment I. TESTING FOR THE PRESENCE OF BIOLOGICAL COMPOUNDS IN FOOD Use the food products you brought to this lab Tests: I.A. BENEDICT’S TEST I.B. LUGOL’S I.C. SUDAN III TEST I.D. BIURET TEST

15. Figure 4. Summary of DNA extraction protocol. II.A. DNA Extraction and Purification II. DNA Extraction, Purification and Quantification

16. Nanodrop spectrometer used to measure DNA concentrations II.B. DNA Quantitation

17. III. CLEAN UP Clean your test tubes with detergent and return them (inverted to dry) to their test tube racks. Return all re-usable supplies to their original location. Disinfect your workstation.