1. Dihybrid crosses and Patterns of inheritance (pedigrees)
Lesson 5.

2. Learning Goals

3. Dihybrid cross genetic diagramP
Phenotypes
Round Yellow seed X
Wrinkled Green seed
(Pure bred)
Genotypes
RRYY
rryy
meiosis
Gametes RY ry
fertilisation F1
RrYy
(Selfed)
Round Yellow
Proportions
100%

4. A dihybrid cross can be treated as two separate monohybrid crosses
The expected probability of each type of seed can be calculated:
Probability of an F2 seed being round = 75% or ¾
Probability of an F2 seed being wrinkled =
Probability of an F2 seed being yellow =
Probability of an F2 seed being green =

5. A dihybrid cross can be treated as two separate monohybrid crosses
The expected probability of each type of seed can be calculated:
Probability of an F2 seed being round = 75% or ¾
Probability of an F2 seed being wrinkled = 25% or ¼
Probability of an F2 seed being yellow = 75% or ¾
Probability of an F2 seed being green = 25% or ¼

6. THE LAW OF INDEPENDENT ASSORTMENT
It appears that the inheritance of seed shape has no influence over the inheritance of seed colour
The two characters are inherited INDEPENDENTLY
The pairs of alleles that control these two characters assort themselves independently

7. Mendel & Meiosis
The pairs of chromosomes could orientate in different ways at Anaphase 1

8. Patterns of Inheritance

9. Pedigrees
A pedigree is a genetic family tree that shows how prevalent a trait is in a family unit from generation to generation.
They are often used to track the expression of genetic conditions and disorders.

10. Pedigrees Squares represent males and circles females.
A coloured in shape means that person has the trait in question.
A half coloured in shape means that they are carrying an allele for a recessive trait.

12. Anatomy of a Pedigree Male (left) Female (right) Affected individuals
Carriers (Heterozygotes for autosomal recessive)
Deceased individuals
Sex unspecified
Male (left) Female (right)

13. Autosomal Dominant Inheritance
Autosomal means not on the sex chromosomes.
Refers to those situations in which a single copy of an allele is sufficient to cause expression of a trait.

14. Autosomal Dominant Inheritance
1. Every affected person should have at least one affected parent.
2. Males and females should be equally often affected.
3. An affected person has at least a 50% chance of transmitting the dominant allele to each offspring.

15. Characteristics of a Dominant Pedigree
An affected individual has at least one affected parent
As a result, dominant traits show a vertical pattern of inheritance
the trait shows up every generation
Two affected individuals may have unaffected children A a
Assume trait isn’t happening spontaneously. How is this possible? (last pt) AA Aa aa A a a 15

16. Autosomal Dominant Inheritance examples
Progeria (caused by a mutation) in which the person ages very rapidly. They die before they can reproduce.
Huntington’s Disease in which the central nervous system starts to break down around the age of 30.

17. Autosomal Recessive Inheritance
Refers to those situations where two recessive alleles result in a trait being expressed.

18. Autosomal Recessive Inheritance
1. An affected person may not have affected parents. Parents would be carriers.
2. Affects both sexes equally. Can appear to skip generations.
3. Two affected parents will have affected children 100% of the time.

19. Characteristics of Recessive Pedigrees
In pedigrees involving rare traits, a horizontal pattern of inheritance is observed
the trait may not appear in every generation
An individual who is affected may have parents who are not affected, particularly as a result of consagineous matings
All the children of two affected individuals are affected a a aa a a

20. Autosomal Recessive Examples
Albinism is a genetic condition which is the loss of pigment in hair, skin and eyes.
Tay Sachs is a genetic disorder which is a build up of fatty deposits in the brain, eventually proving to be fatal.
Cystic Fibrosis is the most common fatal genetic disorder. Mutation in chloride transport protein that causes thick mucus to build up in lungs

21. Pedigree of a family with some members showing Huntington disease

22. Huntington disease is

23. Huntington disease is

24. Genetic Tests Karyotype Fluorescence in situ hybridization (FISH)
Details chromosomal abnormalities through fluorescent tags on chromosomes
Gene testing
Analyzes specific sequence of gene. i.e. breast cancer susceptibility gene BRCA1 and BRCA2
Biochemical testing
Analyzes abnormal enzymes and proteins (Tay Sachs)

25. FISH

26. FISH

27. Genetic Test for Cystic Fibrosis
In 1989 researchers at Sick Kids identified the gene for cystic fibrosis
Gene was on chromosome 7 and named CFTR (cystic fibrosis transmembrane conductance regulator)
Over 1600 possible mutations in CFTR!
Genetic tests can identify mutations % of the time
1 in every 3600 children in Canada are born with CF

28. Genetic test for Huntington disease
In 1983 the gene for Huntington disease called huntingtin was the first human disease-associated gene to be mapped to a chromosome
In 1993 huntingtin gene was sequenced
CAG repeats
Current genetic test looks for CAG repeats (36-39 repeats necessary for disease)

29. Genetic Counselling
If there is a family history of a disease, couples may consult a genetic counsellor
Use pedigrees to determine genotypes of the family members
Explains probability on passing on disease-causing allele to children

30. Issues with genetic screening
Why carry out genetic screening at all?
When is a test accurate and comprehensive enough to be used as the basis for screening?
Once an accurate test becomes available at reasonable cost, should screening become required or optional?
If a screening program is established, who should be tested?
Should private companies and insurance companies have access to employees and client test results?
What education needs to be provided regarding test results?

31. Gene Therapy: A future cure?
Technique aimed at treating genetic disorders by introducing the correct form of the defective gene into a patient’s genome
Copy of “normal” gene is inserted into a vector (usually viral DNA)
Virus infects cells and delivers gene into chromosomes

33. Future of Gene Therapy
Still in experimental stages due to two obstacles:
immune response to viral vector and poor integration into target chromosome
In 2000 St. Michael’s Hospital became performed Canada’s first gene therapy for treatment of heart disease; gene produced protein for stimulation of new blood vessels