1. JODI SCOTT, MS EDUCATION AND PUBLIC RELATIONS ANASTASIA MOSQUITO CONTROL DISTRICT ST. AUGUSTINE, FL The Buzz About Mosquito Control

2. The Good, The Bad and The Funny Looking Good: Male Mosquitoes Food Source Bad: Female Mosquitoes Can Vector Pathogens: WNV Malaria Dengue EEE Dog Heart Worm Funny Looking: Larvae

3. Mosquitoes VS. Bees Size: Very small Habitats: Larvae found in water Adults found foraging around people and plants Activity time: Night Most 1-2 hours after sunset or sun rise Areas of activity: Around water and people/animals Size: 10 to 20 times bigger Habitats: Larvae found in hive Adults found foraging from flowers Activity time: Day Areas of activity: Around plants and vegetative flowers

4. Integrated Mosquito Management (IMM) LarvicidingAdulticiding EducationSource Reduction

5. IMM Source Reduction Education

6. Larviciding Adulticiding IMM

7. Control Methods: Larval Applying Bti: Bacteria/ Stomach poison Source Reduction: Drain & Washout

8. Control Methods: Adults Physical & Mechanical: Screen doors & windows Sticky Traps Attractive Traps Personal Protection: Clothing & Repellents Chemical Control: Permethrin Sumithrin for Ground Dibrom for Air

9. Can The Pesticides Used For Mosquito Control Kill Honey Bees? Yes  If directly applied to honey bees  And in the case of overdoses

10. Does Mosquito Control Kill Honey Bees?  Why? No!

11. MOSQUITO CONTROL LAW (CHAPTER 388) & DAC’S CODE (%E-13): ULV APPLICATION OF PESTICIDE FOR ADULT MOSQUITOES HAS TO BE JUSTIFIED BASED ON: VIRAL CASES OF: HUMANS OR ANIMALS POSITIVE VIRAL CASES OF SENTINEL ANIMALS & MOSQUITOES HIGH NUMBERS DURING LANDING RATE COUNTS AND COLLECTIONS OF MOSQUITOES IN AREAS (>25 MOSQUITOES) SPECIAL CONDITIONS: FLOOD, HURRICANES AND VIRAL OUT BREAKS AMCD’S Adult Mosquito Control Operation:

12. GROUND ULV SPRAYING OF: PERMETHRIN NO SMELL EFFECTIVE 0.0017 OZ PER ACRE AERIAL SPRAYING FOR HOT SPOTS & EMERGENCY SPRAYING: DIBROM 0.5-0.6 OZ PER ACRE BASED ON WEATHER, DISEASE OUTBREAK & NEED FOR IT *WE HAVE A NO FLY LIST AND DO NOT SPRAY CONSERVATION LANDS & FEDERAL/STATE PARKS WITHOUT DISEASE AMCD’S Adult Mosquito Control Operation:

13. ASSESSMENTS OF THE IMPACT OF AERIAL ULV APPLICATIONS OF DIBROM ON HONEY BEES IN MANATEE COUNTY (1999-2000) Research: Dr. Harry Zhong, FAMU

14. Research Location & Hive Set-Up Location: Palmetto, Manatee County, Florida Two Treatment Sites: Terra Ceira Rd & Port Manatee One Control Site: Manatee Fruit at Cortez Rd 16 Bee Hives (in a cluster of 4) were set up at each site 8 Hives in open and 8 hives in forest area at Terra Ceira Rd

15. Sample Collection Dibrom Residue Yarn Samples Naled Concentration In Air Filter Paper Samples Naled Ground Deposition Bee Mortality Dead Bee Trap Screen 1/8 Inch Hardware Cloth With Surface Area 0.19m 2 & Height 25cm *Dead bees collected before and after aerial ULV application

16. Dead bees found in a screened dead bee collector Zhong -- AMCD 2008-2 09/11/2008

17. Zhong -- AMCD 2008-209/11/2008

18. Honey Production (1999 & 2000) 1999 2000 %INCREASE Site 1 Open 52.5 ± 23.7 lbs 73.4 ± 45.8 lbs40% Forest46.8 ± 35.0 lbs98.3 ± 49.9 Ibs 110% Site 2 Open71.2 ± 24.7 lbs107.6 ± 28.7 lbs51% Site 3 (Control Site) Open 88.6 ± 38.4 lbs114.8 ± 38.3 lbs30% Zhong -- AMCD 2008-2 09/11/2008

19. Dibrom Residue Impact on Honeybees Zhong -- AMCD 2008-2 09/11/2008 Bee Mortality was correlated with Naled residue ground deposition Filter Paper Data When Nale residue was less than 1500 μg/m 2, bee mortality was not significantly different between treated and control groups Honey Production was not significantly different between spray and control sites

20. Mosquito Control District Bee Keepers Reduce Naled residue deposits near hives  Inform Bee Keepers when spraying will occur  Continue implementing IMM practices Reduce number of honeybees resting in front of hive during the night  Orient the hive entrance direction to downwind to reduce the exposure Place hive in vegetated area- trees or tall grass- low bee mortality Minimize Honey Bee Mortality

21. Florida Bee Kill Investigation Report 9 Separate bee keepers in 7 counties reported kills between September & November 2011 Investigation by DACS/DPI Initial report by bee keepers implicated mosquito control activities Collected samples from affected bee keepers Inspected pesticide use: mosquito & agricultural operations Collected dead bees, combs & honey, swabbed samples from hive exteriors and materials found in the hives being used for control of bee pests

22. Florida Bee Kill Investigation Report Fipronil in sugar syrup used to feed colonies most likely explanation of the bee kills experienced in Brevard County As the colonies that were exposed to this material declined, foragers from neighboring hives robbed the fipronil-tainted syrup or the honey made from the syrup and experienced bee deaths as well

23. Florida Bee Kill Investigation Report Fipronil in sugar water has been used to eradicate feral bee colonies in New Zealand Bee kills outside of the Brevard /Indian River County area have not been linked to any intentional poisoning or pesticide misuse. Misuse of amitraz for varron mite control, and fipronil containing traps for hive beetle control was observed at a number of locations.

24. Bee Deaths in St. Johns County May 2011 Bee keeper from SR 16 complained that mosquito control spray by airplane killed many of his bees DACS did investigation from April to May No mosquito spray in St. Johns in April and May by aerial Bee keeper saw Jacksonville Mosquito Control airplane fly by his area. Flight was a test run, no chemicals dispensed The case was dismissed in late May

25. THANK YOU FOR YOUR TIME! Contact Information: AMCD 500 Old Beach Rd St. Augustine, FL 32080 904-471-3107 www.amcdsjc.org