1. The Insecticide Resistance Section Of The PopBio Database Emmanuel Dialynas IMBB/FORTH, Greece 18 July 2013

2. The Process Data collection & recording Submission - Curation Presentation

3. What can be recorded? Investigator Food state Catch method Images/Photographs Ιnsecticide & concentration Synergist & concentration Assay method Anatomy parts Environmental conditions & factors Collection period Sample sex Geolocation & coordinates Publication data Sample size Species identification method

4. What results can be recorded? Enzymatic activity - Inhibition rate Permeability Disease transmission Variation Mortality percentage KDT Protein absorbance Mutation frequency Lethal dose Lethal concentration Resistance mechanism

5. Data collection and recording tools Spreadsheet (Libre Office, Excel, Google Docs) ISA-tools & ISA-tab. Desktop applications, standardized output. Android mobile application and web interface in development.

6. Data collection and recording tools: Spreadsheets Most familiar. Easy to add extra fields. Data cannot be fully validated. Libre Office, Excel Work offline. Hard to collaborate. Frequent backup. Google Docs Integrated collaboration. Less frequent backups. Ontology assistant. Easier to get feedback/ support from Curator. Internet conn. required.

7. Data collection and recording tools: ISA-tools Form driven data entry Offline and online Most fields can be validated Ontology assistant Standardized output Frequent backups required Adding fields can be complicated No built in collaboration mechanism

8. Data collection and recording tools: Android application Recording data on the spot Process automation Date, time, location validation Built in image capturing Online/offline + Date, time, coordinates PopBio +

9. Data collection and recording tools: Web interface Online data entry Full data validation Ontology assistant Built in collaboration Offline

10. Data collection and recording tools: Web interface

11. Data searching & presenting Geolocation Year Species Insecticide Catch method Insecticide Assay method Result

12. Data searching & presenting Data provided by ANVR

13. Data searching & presenting Data provided by ANVR All collections in year 2000 All Anopheles arabiensis collections

14. Data searching & presenting Data provided by ANVR All insecticides - all species - all years

15. Data searching & presenting Data provided by ANVR Mortality rates in permethrin assay >95% <=95%

16. Data searching & presenting Lethal time 50 (LT50) in samples from Cameroon and Ghana

17. Giving Back To The Community Training Continuous support IRbase installation at MRTC (Bamako, Mali) for WHO-AFRO

18. Special thanks to: WHO-AFRO, ANVR Janet Hemingway, Louise Kelly Hope, Clare Strode Hillary Ranson, Joseph Burrhani http://www.vectorbase.org info@vectorbase.org Funded by: