1. Organisation Internationale de la Vigne et du Vin Paris 14 mars 2009 ANTICANCER EFFECTS of GRAPE SEED EXTRACTS FROM ITALIA, PALIERI and RED GLOBE CULTIVARS, on HUMAN COLON CANCER CELL LINES Mariano Bizzarri Dept. Experimental Medicine University La Sapienza, Roma

2. AIM of the STUDY 1.CORRELATION between ANALYTE COMPOSITION of both SKIN and SEED EXTRACTS to SPECIFIC CULTIVARS and AGRONOMIC TECHNOLOGIES 2.CELL CYCLE EFFECTS on DIFFERENT CANCER CELL LINES 3.APOPTOTIC EFFECTS ON CANCER CELL LINES 4.RECOGNIZE the APOPTOTIC PATHWAY TRIGGERED by GRAPE SEED or SKIN EXTRACTS

3. CORRELATION between ANALYTE COMPOSITION of both SKIN and SEED EXTRACTS to SPECIFIC CULTIVARS and AGRONOMIC TECHNOLOGIES

4. CULTIVARS : RED GLOBE PALIERI UVA ITALIA

5. LC/MS ANALYSIS of GRAPE SEED EXTRACTS

10. CELL CYCLE EFFECTS on DIFFERENT CANCER CELL LINES

11. CELL LINES CONTROL: NORMAL HUMAN FIBROBLASTS CANCER LINES: BREAST CANCER - MCF7 - MDA-MB231 COLON CANCER - CACO2 - HCT8

12. NORMAL HUMAN FIBROBLASTS

19. APOPTOTIC EFFECTS ON CANCER CELL LINES

26. RECOGNIZE the APOPTOTIC PATHWAY TRIGGERED by GRAPE SEED or SKIN EXTRACTS

27. CACO2 CELLS

33. HCT8 CELLS

39. THE KINASE “CONNECTION”

41. OTHER CANCERS

42. Breast cancer PROLIFERATION CURVES

43. APOPTOSIS

44. WORKING GROUP Dep.t of EXPERIMENTAL MEDICINE University La Sapienza, Roma S. Dinicola A. Cucina A. Pasqualato F. D’Anselmi S. Proietti M. Bizzarri Dep.t of CHEMISTRY University La Sapienza, Roma A. Laganà C. Cavaliere Dep.t of VEGETAL BOTANICS University La Sapienza, Roma G. Pasqua A.R. Santamaria CRA-UTV Agricultural Research Council - Research Unit for table grapes and wine growing in Mediterranean environment - Bari D. Antonacci