1. Artificial Olfactory Systems (AOS) or electronic nose ISENose 2000 www.soatec.unipr.it www.ssmg.unipr.it

2. What is an artificial olfactory system (AOS)? An AOS is an instrument composed of a sensor array and of a suitable data treatment software, able to characterize and recognize simple and complex odours This technology does not identify the single odour components and their relative quantities, but it gives a complete analysis, operating in a similar way to the human olfactive system The principle advantages of using the AOS are:  Simplicity and synthetic assessment  Immediate result  No sample pretreatment

3. Artificial versus natural olfactory system

4. ISE Nose2000 AOS operating system SAMPLE SENSORS FINGERPRINT SOFTWARE DFA RESULT

5. Optimized for “QUALITY CONTROL” applications AOS with MOS sensors Packaging quality control Evaluation and control of raw materials Olive oil defects Paperboard quality control Parmigiano Reggiano cheese quality control PRACTICAL APPLICATIONS

6. Main application: quality controlfor food packaging Deep Knowledge of the Problem, acquired within the frame of cooperation with Barilla Alimentare S.p.A. Optimisation of Hardware configuration and Software procedures for Quality Control application Software Processing module based on creation and maintenance of a dynamic Data Base of reference samples (“Bad” and “Good” samples) Fully Assisted operation and classification procedure

7. 1.ISENOSE 2000 main unit 2.Self-sampling unit 3.Supervision PC (Wintel configuration) The “ISENose2000” Artificial Olfactory Device The system is composed by:   

8. ISENose2000 Technology The ISENose2000 system is based on MOS thick- and/or thin-film chemical gas sensors Air Flow in Air Flow out Humidity sensor Temperature sensor Metal-Oxide Gas Chemical Sensors Sensors chamber

9. 16-Position Self-sampler Proprietary Design Inexpensive, easy-to-use Optimized for Quality Control activity on sample batches Completely SW-driven sample selection, data acquisition and cleaning of the line

10. Detail of vial connection Each pair of needles is used for one vial where a given sample of the desired material has been inserted

11. Inside the nose: the MOS sensors chamber

12. Top view TEMPERATURE SENSOR HUMIDITY SENSOR AIR INLET AIR OUTLET MOS SENSORS

13. Working principle of MOS sensors ½ O 2 + (SnO 2-X )* O _ (SnO 2-X ) CO + O _ (SnO 2-X ) CO 2 + (SnO 2-X )*

14. SENSOR DATA TO THE PC SENSORS CHAMBER MASS FLOW CONTROLLER INPUT: GC AIR SOURCE 16 SAMPLE VIALS TUBING CONNECTORS OUTPUT: EXTERNAL AIR Air flow control system 4-Ways Valve 16 Ways Valve (Autosampler) 4 2 1 3 Serial connection between PC and ISENOSE main unit PC Schematic of operation

15. Application software installation Typical (Data acquisition, visualization, processing with Statistical tools) Quality Control Ready-to-use application for classification and recognition of samples in Q.C. applications

16. Program opening page

17. Method module

18. Channel description

19. Analysis in progress

20. Fingerprint

21. DFA Analysis represented by poligonals

22. 3D View