1. Silvia Maria DOGLIA Nanoparticelle di aggregati proteici DIPARTIMENTO DI BIOTECNOLOGIE E BIOSCIENZE

2. Biophysical approaches for nanomedicine Single-molecule biophysics to detect conformational states of a single molecule such as misfolded protein during aggregation. Advanced microscopic techniques to probe at molecular level the interaction of nanoparticles within complex biological systems (cells, tissue and organs). An important requirement for nanomedicine is that these techniques must be rapid, inexpensive and non invasive for in vitro and in vivo diagnostics. Among these techniques: FT-IR microspectroscopy

3. FT-IR study of protein aggregate nanoparticles Possible prion strain identification in tissues containing PrP Sc deposits - brain and lymphoid organs (Kretlow et al. BBA 2006). Protein aggregation in recombinant bacteria for the production of heterologous proteins in form of inclusion bodies (IBs). IBs can be internalised by eukaryotic cells. Biologically active molecules, trapped within IBs, can be released in the target cells (Gonzalez-Montalban et al. Microbial Cell Factories 2006).