1. Assessment of the Safety and Efficacy of Hemp for Poultry Diets J.D. House, E. Goldberg, N. Gakhar, M. Aliani Department of Human Nutritional Sciences University of Manitoba 11/17/20102011 CHTA National Hemp Convention

2. Outline Current Status of Hemp Use in Livestock Rations – Canada – European Union Preliminary Studies with Laying Hens Canadian Agriculture Adaptation Program Grant – Partnership between CHTA and University of Manitoba – Develop data in support of safety and efficacy of hemp for poultry rations 2011 CHTA National Hemp Convention

3. Hemp Products: Food and Feed Ingredients Hemp Seed Hemp Nuts Hemp Oil DM%Fat%Protein%NDF%Ash%GE (MJ/kg) Hemp Seeds94.130.424.032.14.824.2 Hemp Nuts95.146.735.97.86.425.3 Hemp Protein Flour95.110.240.730.56.720.4 Hemp By-Product94.910.312.764.93.920.2 House et al., 2010. J. Agric. Food Chem.58: 11801-11807

4. Fatty Acid Profile of Hemp Oil Fatty Acid% of TotalStd. Dev. PalmiticC16:05.900.52 PalmitoleicC16:10.120.03 StearicC18:02.610.22 OleicC18:112.211.91 LinoleicC18:255.860.55 Gamma-LinolenicC18:3 n-63.170.60 Alpha - LinolenicC18:3 n-317.271.77 ArachidicC20:00.740.08 StearidonicC18:41.180.29 GadoleicC20:10.380.03 BehenicC22:00.310.04 LignocericC24:00.140.03 NervonicC24:10.110.04 Varietal differences in Fatty Acid Content of Hemp. Przybylski, R. Unpublished

5. Alpha-Linolenic Acid Content of Plant Oils

6. Amino Acid Composition of Hemp Seed (mg/g Protein) House et al., 2010. J. Agric. Food Chem.58: 11801-11807

7. Hemp as a Feed Ingredient Summary Whole hemp seed offers high protein and energy content, making it attractive from a feed formulation standpoint High levels of ALA offer potential for hemp seed and oil to be used for the production of omega-3 eggs and meat Challenge: – Hemp is not an approved feed ingredient in Canada 2011 CHTA National Hemp Convention

8. Excerpt from the Regulatory Guidance (RG-1) – Canadian Food Inspection Agency “In Canada, livestock feeds are regulated, as per the federal Feeds Act and Regulations, by the CFIA. All single ingredient feeds manufactured, sold or imported into Canada must be approved and listed in either Schedule IV or Schedule V of the Feeds Regulations. At the present time, hemp products are not approved as livestock feed ingredients in Canada. Each hemp product intended to be used as a livestock single ingredient feed (e.g., hemp meal, hemp oil, seeds) will require separate approval.” http://www.inspection.gc.ca/english/anima/feebet/regdir/regdire.shtml

9. Process for Registration of Hemp Seed Products Regulatory Guidance Process Administrative information – Labeling – Ingredient Description – Ingredient Composition – Manufacturing Process – Safety Data Requirements – Efficacy Data Requirements Minimum of 3 studies required http://www.inspection.gc.ca/english/anima/feebet/regdir/sect3_10e.shtml

10. Hemp in Livestock Rations 2011 CHTA National Hemp Convention

11. Hemp in Livestock Rations 2011 CHTA National Hemp Convention

12. Nutrition trials with Layers Silversides and Lefrancois, 2005, British Poultry Science, 46(2) ; 231-235 Diet (g Hemp Seed Meal/kg diet)

13. Situation in Europe 2011 CHTA National Hemp Convention

14. Towards Registration in Canada Safety and Efficacy Studies Funding from the following is committed to addressing the need for data: – Canadian Hemp Trade Alliance – Poultry Industry Council – Manitoba Innovation, Energy and Mines STIC Fund – Canadian Agriculture Adapation Program (AAFC) 2011 CHTA National Hemp Convention

15. Studies to Date 2011 CHTA National Hemp Convention

16. Study Design 48 Bovan hens – 19 weeks of age Housed in individual metabolism cages for 12 weeks Wheat/Barley/Soy-based diets – Formulated to meet hen requirements 48 Bovan hens (19 weeks) Hempseed oil (4, 8, 12%) Hempseed (10, 20%) Control

17. Study Design Measurements – Production – Egg quality – Fatty acid analysis – Indices of bird health and welfare – Sensory analysis of cooked eggs

18. Results – Hemp Seed (HS) 2011 CHTA National Hemp Convention

19. Results – Hemp Oil (HO) 2011 CHTA National Hemp Convention

20. Results Omega-3 Fatty Acids 2011 CHTA National Hemp Convention

21. Sensory Evaluation Sensory analysis conducted with trained panel – Cooked egg product – Attributes included aroma and flavour No significant differences on sensory parameters due to hemp inclusion 2011 CHTA National Hemp Convention

22. Next Steps Complete studies with laying hens Initiate and complete studies with broiler chickens Project completion: March 31, 2014. 2011 CHTA National Hemp Convention